Evidence map›Paper›PMID 41044762›Full record

ArticleMicrobial cell factories2025

Scaling up the production of fungal perylenequinones and investigating their biosynthesis through stable isotope labeling.

Reema A Al-Qiam, Sachin Dumbare, Tyler N Graf, Warren S Vidar, Huzefa A Raja, Cedric J Pearce, Shabnam Hematian, Nicholas H Oberlies

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Reema A Al-QiamDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Sachin DumbareDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Tyler N GrafDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Warren S VidarDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Huzefa A RajaDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Cedric J PearceMycosynthetix, Inc., Hillsborough, NC, 27278, USA.
Shabnam HematianDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Nicholas H OberliesDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA. n_oberli@uncg.edu.

Funding

Project 3:Isolation Chemistry of Filamentous Fungi and Biological EvaluationP01CA125066 · NCI · OHIO STATE UNIVERSITY · PI JIMMY ORJALA · 2007 to 2026
$27.2M
NCI NIH HHS P01 CA125066U.S. Department of Energy DE-SC0024433
6 · The paper itself

Abstract

backgroundPerylenequinones, such as hypocrellins and hypomycins, are fungal secondary metabolites with potential for pharmaceutical and industrial applications due to both their physical and biological properties. This study focused on their sustainable production. Additionally, stable isotope labeling was used to probe the biosynthesis of these compounds, demonstrating how sugars are likely incorporated into the perylenequinone scaffold.

methodsShiraia sp. (strain MSX60519; Shiraiaceae, Pleosporales) was cultivated under varying nutrient conditions to evaluate the production of perylenequinones, with sugars serving as primary carbon sources. Five metabolites were isolated (from oatmeal cultures) using environmentally friendly solvent-based techniques, and the process was further optimized to maximize yields. High-performance liquid chromatography (HPLC) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) were employed to detect, characterize, and quantify the major compounds. Furthermore, feeding experiments were performed using

resultsThis study yielded three key findings. First, the production of perylenequinones was significantly enhanced by supplementing fermentation media with sugars, and disaccharides significantly enhanced the production of perylenequinones compared to monosaccharides. Optimizing sugar concentrations during the fermentation further influenced the profile of secondary metabolites. Second, stable isotope labeling experiments confirmed that sugars are the primary building blocks of perylenequinones, as noted by tracing

conclusionThese findings provide critical insights into optimizing fermentation conditions for the scaled and sustainable production of perylenequinones. This approach offers a cost-effective and environmentally friendly pipeline for harnessing these valuable compounds, paving the way for broader pharmaceutical and industrial applications.

Indexed as

AscomycotaIsotope LabelingPeryleneQuinonesCarbon IsotopesChromatography, High Pressure LiquidFermentationCarbon IsotopesPeryleneQuinones13C-labeledCarbon metabolismGreen chemistryHypocrellinsHypomycinsShiraiaceaeShiraia sp.Stable isotope labelingSustainable production

Identifiers

PMID41044762
PMCPMC12492911

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.