Evidence map›Paper›PMID 41044339›Full record

ArticleScientific reports2025

Digital PCR-Based characterization of a Zhonghuang 6106 soybean genomic DNA reference material for its food and feed detection.

Cheng Wang, Ruihuan Li, Na Liu, Xin Qi, Chao Xu, Junjin Yao, Chen Zhang, Yasi Wang, Qingkuo Lan, Yong Wang and 1 more

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Cheng Wang *Tianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Ruihuan Li *Tianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Na LiuTianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Xin QiTianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Chao XuTianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Junjin YaoTianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Chen ZhangTianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Yasi WangTianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Qingkuo LanTianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Yong WangTianjin Academy of Agricultural Sciences, Tianjin, 300192, China.
Xin ZhaoTianjin Academy of Agricultural Sciences, Tianjin, 300192, China. zhaoxin2008999@126.com.

Funding

Development and application support system construction of molecular characteristic safety detection technology in the major national science and technology projects 2022ZD04020Development of standard materials for testing transgenic products in major special projects for the cultivation of transgenic new varieties 2016ZX08012003
6 · The paper itself

Abstract

The genomic DNA reference material of genetically modified soybean Zhonghuang6106, developed in this study, was prepared by extracting genomic DNA from the leaves of both homozygous transgenic soybean Zhonghuang 6106 and non-transgenic soybean Zhonghuang10, followed by proportional mixing of the genomic DNA. Eight qualified laboratories independently validated the certified reference material (CRM) using the digital polymerase chain reaction (dPCR) method. The certified value for the transgene-specific sequence copy number concentration was determined to be (1.04 ± 0.16)×10³ copies·µL

Indexed as

Animal FeedDNA, PlantGenome, PlantGlycine maxPlants, Genetically ModifiedPolymerase Chain ReactionReference StandardsDNA, PlantCertified reference materialDigital PCRHomogeneityZhonghuang 6106 soybean

Identifiers

PMID41044339
PMCPMC12494736

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.