ArticleNature methods2025
Spatiotemporal focusing enables all-optical in situ histology of heterogeneous tissue.
Article in Nature methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 authors.
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Abstract
Living systems embody heterogeneous tissues with complex opto-mechanical properties. Achieving organ-scale, diffraction-limited volumetric imaging that faithfully captures in vivo architecture requires minimizing sample deformation and preserving vascular and neuronal continuity across delicate tissue interfaces. As a solution to this problem, we developed a robotic nonlinear optical system for iterative multiphoton microscopy and opto-micromachining. Adaptive control enabled days-long autonomous operation, while spatiotemporal line-focused ablation increased the machining efficiency by 100-fold over prior configurations. Using the intact murine craniocerebral system as a test bed, our approach demonstrates the potential for whole-body submicrometer resolution imaging and anatomical reconstruction.
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