Evidence map›Paper›PMID 41043429›Full record

ArticleStem cell reports2025

Retinoic acid signaling guides the efficiency of inner ear organoid-genesis and governs sensory-nonsensory fate specification.

R Keith Duncan, Liqian Liu, Mo Moyer, Andrew Wylie, Ranya Dano, Luis Cassinotti

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Retinoic acid in health and disease.Signal transduction and targeted therapy · 2026
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

R Keith DuncanDepartment of Otolaryngology - Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan, Ann Arbor, MI, USA; Veterans Affairs Ann Arbor Health System, Ann Arbor, MI, USA. Electronic address: rkduncan@umich.edu.
Liqian LiuDepartment of Otolaryngology - Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan, Ann Arbor, MI, USA.
Mo MoyerDepartment of Otolaryngology - Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan, Ann Arbor, MI, USA.
Andrew WylieDepartment of Otolaryngology - Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan, Ann Arbor, MI, USA.
Ranya DanoDepartment of Otolaryngology - Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan, Ann Arbor, MI, USA.
Luis CassinottiDepartment of Otolaryngology - Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan, Ann Arbor, MI, USA.

Funding

RRD VA I01 RX003400
6 · The paper itself

Abstract

Inner ear organoid development-from germ layer to otic vesicle (OV) formation-relies on timed chemical cues to recapitulate major signals in vivo. In contrast, later stages of differentiation-from OV to organoid formation-are self-guided, even though these stages are modulated by several key morphogens in vivo. We sought to elucidate additional morphogens that might improve culture efficiency and influence cell fate decisions. Using a whole-transcriptomic approach, we identified major differences in native and stem-cell-derived OVs related to anterior-posterior patterning and retinoic acid (RA) signaling. Increasing the level of RA during OV formation in these cultures modulated organoid efficiency, increased nonsensory markers, decreased sensory markers, and decreased hair cell production. The organoid culture platform mimics the exquisite RA sensitivity found in normal inner ear development and provides a tunable system for generating sensory and nonsensory cell types in inner ear organoids.

Indexed as

Ear, InnerOrganogenesisOrganoidsSignal TransductionTretinoinAnimalsCell DifferentiationCell LineageGene Expression Regulation, DevelopmentalMiceTretinoinanterior-posterior axisembryonic stem cellfate specificationhair cellsinner ear organoidretinoic acidRNA-seqTGF-β

Identifiers

PMID41043429
PMCPMC12790742

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.