Evidence map›Paper›PMID 41043034›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2026

Combination of 3D Micro Contact Printing and Scraping Technique for Site Selective Cocultivation Across a Porous Micro Structured Scaffold.

Dana Brauer, Anni Peng, Justyna Borowiec, Jörg Hampl, Shannon Prehl, Maren Klett, Merle Johanna Küstner, Frank Weise, Andreas Schober, Sukhdeep Singh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Dana BrauerDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.ORCID https://orcid.org/0009-0005-9445-0524
Anni PengDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.
Justyna BorowiecDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.
Jörg HamplDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.
Shannon PrehlDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.
Maren KlettDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.
Merle Johanna KüstnerDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.
Frank WeiseDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.
Andreas SchoberDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, P.O. Box, 98684, Ilmenau, Germany.
Sukhdeep SinghResearch Group Bioorganic Chemistry of Bioactive Surfaces, Institute of Chemistry and Biotechnology, Prof-Schmidt-Straße 26, 98693, Ilmenau, Germany.ORCID https://orcid.org/0000-0001-6871-4294

Funding

AiF Projekt KK5240403NK1Bundesministerium für Bildung und Forschung FKZ 03VP08691Carl-Zeiss-Stiftung CZS: P2022-00-135
6 · The paper itself

Abstract

3D-microcontact printing (3D-µCP) technique combines the advantages of microcontact printing and microthermoforming for the fabrication of functional biomaterials with complexity closer to real tissue. Despite its unmet advantages in terms of complexity and processability in a single step, this technique is limited to the front side of the substrate. Considering the advantage of inherent topography patterns on the reverse side of the substrate, an additional degree of patterning can be envisioned. However, selective patterning on the reverse side is challenging due to the fragility of the cell culture on the front side. Herein, a technically simple scraping technique is presented in combination with 3D-µCP to generate 3D cell patterns on both the front and the reverse side of a micro thermoformed substrate. The technical advancement of 3D-µCP with the scraping technique offers a complex, multi-layered co-culture system that can be used to study cell-to-cell crosstalk at the microlevel with topographies similar to real tissue. Direct seeding of the third cell type on the scaffold expands cell type complexity, resulting in a tri-culture model. As a proof of concept, a triculture of EA.Hy 926 endothelial cells, HepG2 hepatoblastoma cells, and NIH3T3 fibroblasts are generated, mimicking the tumor microenvironment in the liver.

Indexed as

Printing, Three-DimensionalTissue ScaffoldsAnimalsCoculture TechniquesHumansMicePorosityTissue Engineeringalpha‐fetoproteincocultivationfunctional biomaterialsliver sinusoidmicrocontact printingmicropatterning techniquetumor microenvironment

Identifiers

PMID41043034
PMCPMC12934357

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.