Evidence map›Paper›PMID 41042493›Full record

ArticleAMB Express2025

Effects of quadric probiotic blends on rumen fermentation, nutrient degradability, and methane emission in sheep: an in vitro study.

Ali S A Saleem, Khaled M Al-Marakby, Mohamed Y Elaref, Sabry M Bassiony, Amera A Helal, Usama M Abdel-Monem, Sameh A Abdelnour

Abstract read
In one paragraph

Article in AMB Express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Dietary Supplementation with Rumen-DerivedAnimals : an open access journal from MDPI · 2026
    Article
  2. Review
  3. Dietary Supplementation ofMicroorganisms · 2026
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ali S A SaleemAnimal Production Department, Faculty of Agriculture, Sohag University, Sohag, Egypt. ali.saleem1706@gmail.com.ORCID http://orcid.org/0009-0007-3258-0256
Khaled M Al-MarakbyAnimal Production Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Mohamed Y ElarefAnimal Production Department, Faculty of Agriculture, Sohag University, Sohag, Egypt.
Sabry M BassionyAnimal Production Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Amera A HelalAnimal Production Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Usama M Abdel-MonemAnimal Production Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Sameh A AbdelnourAnimal Production Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt. samehtimor86@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The considerable contribution of ruminant livestock to methane emissions has become a major global concern in recent years. Although dietary approaches for reducing ruminant methane emissions have been explored, the sustainable potential of probiotics to influence rumen function and lower methane production has increasingly attracted research attention. While previous studies have focused on single or dual-strain probiotics, this study is among the first to evaluate the synergistic effects of quadric-strain formulations. Hence, this study aimed to evaluate the impact of multi-strain probiotic blends, each at two distinct concentrations on rumen fermentation, nutrient degradability, and methane emission in sheep using an in vitro gas production technique following a completely randomized design. The basal diet with no probiotic supplements served as a control, while the supplemented bacterial combinations were Bacillus licheniformis, Lactobacillus acidophilus, L. bulgaricus, and Bifidobacterium bifidum (ABLB; at a ratio of 1:1:1:1) at levels of 2 × 10

Indexed as

Gas productionIn vitro rumen fermentationMethane emissionsNutrient degradabilityPredictive valuesProbiotic combinations

Identifiers

PMID41042493
PMCPMC12494527

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.