ArticleAnalytical and bioanalytical chemistry2025
A two-signal aptasensor for sensitive exosome detection via rolling circle amplification and G-quadruplex structure.
Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Exosomes play a pivotal role in cancer diagnosis and therapy, yet their clinical application faces significant technical challenges. To address this, we developed a sensitive, aptamer-based platform that integrates nucleic acid amplification and a G-quadruplex (G4) with two-signal output for exosome detection. Our approach employs CD63 antibody-conjugated magnetic beads (MBs) for efficient exosome capture and purification. The captured exosomes are then bound by CD63-specific aptamers embedded in rolling circle amplification (RCA) products, forming a sandwich complex with the MBs. The RCA products also contain G4 repeats, enabling two-signal output through interaction with Fe(III)-protoporphyrin IX (Hemin) and N-methylporphyrin-dipropionic acid IX (NMM). The G4-Hemin complex catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), producing a colorimetric signal, while the G4-NMM enhances fluorescence emission. This detection system achieves high sensitivity, with detection ranges of 4.0 × 10
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