ArticleAnalytical chemistry2025
One Assay, Nine Targets: Advancing Viral Surveillance with Multiplex RT-ddPCR.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Five years of wastewater surveillance reveal COVID-19-Associated amplification and seasonal shifts inOne health (Amsterdam, Netherlands) · 2026Article
- Development of Pentaplex Reverse Transcription Droplet Digital PCR Assay for Simultaneous Detection and Absolute Quantification of HIV-1, HIV-2, HCV, and HBV With Internal Control.Electrophoresis · 2026Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral infections continue to pose a major global health challenge, driven by factors such as population growth, migration, and environmental change, all of which contribute to the emergence and reemergence of infectious viruses. Advances in technology now enable the detection of multiple targets from a limited sample volume; however, few studies have fully leveraged these capabilities. In this study, we developed and analytically validated a highly sensitive and specific 9-plex one-step RT-ddPCR assay for the detection of high-risk viruses, including SARS-CoV-2 (N1 and N2 genes), Influenza A and B, Respiratory Syncytial Virus, Hepatitis A and E, along with both endogenous and exogenous controls. Initial validation was conducted using synthetic DNA, followed by application to 38 wastewater samples─complex and heterogeneous matrices that often harbor multiple viral targets. The assay demonstrated excellent analytical performance in terms of sensitivity, linearity, specificity, and reproducibility with detection limits ranging from 1.4 to 2.9 copies/μL depending on the viral target. A direct comparison with singleplex ddPCR assays revealed high concordance (Mann-Whitney test,
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