An Improved Cysteine-Based Labeling Strategy for GPCR Structural Dynamics Studies.

Jintao Liu, Ju Yang, Zhuoqi Wang, Changwen Jin, Yunfei Hu

Abstract read

In one paragraph

Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

5 authors.

Jintao LiuBeijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering & Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871, China.

Ju YangState Key Laboratory of Magnetic Resonance Spectroscopy and Imaging, National Center for Magnetic Resonance in Wuhan, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, China.

Zhuoqi WangBeijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering & Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871, China.

Changwen JinBeijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering & Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871, China.ORCID https://orcid.org/0000-0002-1352-2540

Yunfei HuState Key Laboratory of Magnetic Resonance Spectroscopy and Imaging, National Center for Magnetic Resonance in Wuhan, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, China.ORCID https://orcid.org/0000-0002-0156-2326

Funding

Science Fund for Distinguished Young Scholars of Hubei Province 2025AFA112Strategic Priority Research Program of the Chinese Academy of Sciences XDB0540000

6 · The paper itself

Abstract

Spectroscopic investigations of the dynamic signaling process of G protein-coupled receptors (GPCRs) often require cysteine-based labeling. However, the presence of a highly conserved but labile disulfide bond in the receptor extracellular domain could result in strong background signals, hindering the application of spectroscopic methods in studying GPCR dynamics. Herein we report an improved strategy for site-specific labeling of muscarinic acetylcholine receptors by using phenylarsine oxide (PAO) to reversibly protect the conserved disulfide bond. This method can efficiently reduce background signals while maintaining receptor functionality, enabling both fluorophore and

Indexed as

CysteineReceptor, Muscarinic M2Receptors, G-Protein-CoupledDisulfidesFluorescence Resonance Energy TransferHumansMagnetic Resonance SpectroscopyCysteineDisulfidesReceptor, Muscarinic M2Receptors, G-Protein-Coupleddisulfide bonddynamicsG protein‐coupled receptorlabelingmuscarinic receptor

Identifiers

PMID41041959

DOI10.1002/chem.202501656

PMCPMC13411485

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.