Evidence map›Paper›PMID 41041903›Full record

ArticleArtificial organs2026

Biliary Glutathione as a Potential Marker for Viability Assessment of Cholangiocytes During Normothermic Machine Perfusion of Livers.

Christina Bogensperger, Hannah Esser, Florian Ponholzer, Benno Cardini, Julia Hofmann, Franka Messner, Rupert Oberhuber, Thomas Resch, Dietmar Öfner, Stefan Schneeberger and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Artificial organs, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Christina BogenspergerDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.ORCID https://orcid.org/0000-0002-4129-8670
Hannah EsserDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.
Florian PonholzerDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.ORCID https://orcid.org/0000-0003-3486-7166
Benno CardiniDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.
Julia HofmannDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.
Franka MessnerDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.
Rupert OberhuberDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.
Thomas ReschDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.
Dietmar ÖfnerDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.
Stefan SchneebergerDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.
Annemarie WeissenbacherDepartment of Visceral, Transplant and Thoracic Surgery, organLife Organ Regeneration Center of Excellence, and Daniel Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria.ORCID https://orcid.org/0000-0002-0582-1815

Funding

Medical University of Innsbruck 2018-01-009
6 · The paper itself

Abstract

backgroundNormothermic machine perfusion (NMP) is increasingly used to assess liver grafts before transplantation. While viability assessment of the hepatocellular compartment has been widely adopted, viability assessment of the biliary compartment remains controversial due to the lack of reliable markers. Glutathione (GSH) plays a key role in bile formation and may serve as a marker for biliary viability. Thus, the present study aimed to investigate the suitability of GSH as a potential marker for biliary viability assessment during NMP.

methodsBetween 2018 and 2021, livers undergoing NMP were included in the study. Bile samples were collected at 1 h, 6 h, and at the end of NMP, and then analyzed using enzyme-linked immunosorbent assay (ELISA). Results were correlated with perfusion parameters and clinical outcomes.

results56 livers underwent NMP during the study period, of which 41 were successfully transplanted (73.2%). Within 1 year after transplantation, 19 patients (46.3%) developed biliary complications. 1-year patient survival was 80.5% and 1-year graft survival (death-censored) was 94.6%. The GSH in transplanted livers showed a significant increase over time (Hour 1: 25.9 ± 31.2 μM vs. End: 108.7 ± 95.3 μM, p < 0.001) and significant inverse correlation with the occurrence of biliary complications after transplantation (p < 0.05). GSH concentration at the first hour measurement was significantly lower in livers that were deemed non-transplantable (11.2 μM vs. 25.9 μM, p = 0.006).

conclusionsOur data show an increase of GSH in bile during liver ex situ NMP. We found a negative correlation between GSH concentration and the development of biliary complications, suggesting GSH as a new marker for biliary viability assessment.

Indexed as

BileGlutathioneLiverLiver TransplantationOrgan PreservationPerfusionAdultAgedBiomarkersFemaleGraft SurvivalHumansMaleMiddle AgedBiomarkersGlutathionebiliary complicationsliver preservationliver regenerationnormothermic machine perfusionviability assessment

Identifiers

PMID41041903
PMCPMC12993255

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.