Evidence map›Paper›PMID 41040984›Full record

ArticleFrontiers in cellular and infection microbiology2025

Rapid identification of major

Yuanwu Zou, Zhuo Wang, Zihan Wei, Guanghong Bai, Xiaolin Wang, Shaoyi Qu, Guowei Zhong, Yanbin Gao

Abstract read
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yuanwu Zou *Department of Clinical Laboratory, Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.
Zhuo Wang *Department of Clinical Laboratory, Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.
Zihan WeiDepartment of Clinical Laboratory, Shaanxi Provincial People's Hospital, Xi'an, China.
Guanghong BaiDepartment of Clinical Laboratory, Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.
Xiaolin WangDepartment of Clinical Laboratory, Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.
Shaoyi QuDepartment of Clinical Laboratory, Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.
Guowei ZhongTuberculosis Medicine Department II, Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.
Yanbin GaoShaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Rapid and precise identification of Methods: We developed a multiplex loop-mediated isothermal amplification (LAMP) assay targeting newly identified species-specific genomic markers for simultaneous detection of Results: The assay showed high analytical sensitivity, with limits of detection ranging from 76.013 CFU/mL (95% CI: 60.329-113.924 CFU/mL) for MTBC to 166.602-690.629 CFU/mL for NTM species. All reference strains were correctly identified with no cross-reactivity. Among the clinical isolates, all targeted species were accurately detected. One isolate misidentified as Conclusion: This multiplex LAMP assay offers a rapid, accurate, and field-deployable tool for species-level identification of MTBC and major NTM pathogens. Its simplicity, stability, and compatibility with low-resource settings support its application in routine diagnostics and decentralized tuberculosis programs.

Indexed as

Molecular Diagnostic TechniquesMycobacterium Infections, NontuberculousNontuberculous MycobacteriaNucleic Acid Amplification TechniquesDNA, BacterialGenome, BacterialHumansMycobacterium tuberculosisSensitivity and SpecificitySpecies SpecificitySputumDNA, Bacterialclinical validationloop-mediated isothermal amplificationmycobacterium tuberculosisnontuberculous mycobacteriaspecies identification

Identifiers

PMID41040984
PMCPMC12484021

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.