Evidence map›Paper›PMID 41040718›Full record

ArticlemedRxiv : the preprint server for health sciences2025

Reconstructing pathogen-specific antibody binding epitopes and age-dependent immune signatures from proteomic-scale peptide libraries.

Everlyn Kamau, Nikolina Walas, Minlu Zhang, Kathy Kamath, Jack Reifert, John Shon, Shahjahan Ali, Md Ziaur Rahman, Abul K Shoab, Syeda L Famida and 10 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Everlyn KamauFrancis I. Proctor Foundation, University of California, San Francisco, California, USA.ORCID 0000-0003-4285-2255
Nikolina WalasSchool of Public Health, University of California, Berkeley, California, USA.
Minlu ZhangSerimmune Inc., California, USA.
Kathy KamathSerimmune Inc., California, USA.
Jack ReifertSerimmune Inc., California, USA.
John ShonSerimmune Inc., California, USA.
Shahjahan AliDepartment of Epidemiology, Colorado School of Public Health, Aurora, Colorado, USA.
Md Ziaur RahmanEnvironmental Health and WASH, Health System and Population Studies Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Abul K ShoabEnvironmental Health and WASH, Health System and Population Studies Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Syeda L FamidaEnvironmental Health and WASH, Health System and Population Studies Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Salma AktherEnvironmental Health and WASH, Health System and Population Studies Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Md Saheen HossenEnvironmental Health and WASH, Health System and Population Studies Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Palash MutsuddiEnvironmental Health and WASH, Health System and Population Studies Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Mahbubur RahmanEnvironmental Health and WASH, Health System and Population Studies Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Andrew N MertensDivision of Epidemiology, University of California, Berkeley, California, USA.
Richelle C CharlesMassachusetts General Hospital, Harvard Medical School, Harvard T.H. Chan School of Public Health, Boston, USA.
Daniel T LeungDivision of Infectious Diseases, Department of Internal Medicine, University of Utah, Salt Lake City, USA.
Stephen LubyDivision of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, USA.
Audrie LinDepartment of Microbiology and Environmental Toxicology, University of California, Santa Cruz, California, USA.
Benjamin F ArnoldFrancis I. Proctor Foundation, University of California, San Francisco, California, USA.

Funding

Serologic measures of enteric pathogen transmission for intervention studies and population monitoring in low-resource settingsR01AI166671 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Benjamin F Arnold · 2022 to 2026
$2.7M
NIAID NIH HHS R01 AI166671
6 · The paper itself

Abstract

Public health interventions involving improved water, sanitation and promotion of hygiene behaviors (WASH) plus nutrition, were implemented in Bangladesh in a large randomized controlled trial to assess impact on childhood enteric infection and diarrheal disease. Here, we evaluated magnitude and breadth of humoral responses to enteric pathogens in a subset of children among those received intervention (n=60) versus a control group (n=60) using an integrated method of bacterial display peptide library screening, next-generation sequencing and computational analysis to characterize individual antibody repertoires in serum collected at median ages of 3, 14 and 28 months. We determined high seroprevalence for enteric infections and show that antibody recognition of the putative epitopes and antigenic regions remained consistent over time. With mathematical models, we inferred waning of maternal immunity, and a subsequent immunity boost due to infection. Random peptide library screening has potential utility for rigorous analysis of antibody responses and identification of epitopes indicative of protective humoral immune responses.

Identifiers

PMID41040718
PMCPMC12486044

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.