Evidence map›Paper›PMID 41040332›Full record

ArticlebioRxiv : the preprint server for biology2025

Dosa: A method to covalently barcode proteins for high throughput biochemistry.

Yashwanth Ashok, Kerry L Bubb, Curran Oi, Sayeh Gorjifard, Josh T Cuperus, Christine Queitsch, Stanley Fields

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Yashwanth AshokDepartment of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195.ORCID 0000-0002-5693-6372
Kerry L BubbDepartment of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195.
Curran OiDepartment of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195.ORCID 0000-0003-0911-0292
Sayeh GorjifardDepartment of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195.
Josh T CuperusDepartment of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195.ORCID 0000-0002-8019-7733
Christine QueitschDepartment of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195.ORCID 0000-0002-0905-4705
Stanley FieldsDepartment of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195.ORCID 0000-0001-5504-5925

Funding

Technology to understand genetic variant effects in contextRM1HG010461 · NHGRI · UNIVERSITY OF WASHINGTON · PI Douglas M Fowler, Bruce Colston Trapnell · 2019 to 2026
$18.9M
High-throughput biochemistry with RNA-barcoded proteinsR21HG013504 · NHGRI · UNIVERSITY OF WASHINGTON · PI ASHOK, YASHWANTH, FIELDS, STANLEY · 2024 to 2025
$428k
NHGRI NIH HHS R21 HG013504NHGRI NIH HHS RM1 HG010461
6 · The paper itself

Abstract

Deep mutational scanning couples a protein's activity to DNA sequencing for high throughput assessment of the effects of all single amino acid substitutions, but it largely uses indirect assays, like growth, as proxy for protein activity. Here, we covalently link variant proteins

Indexed as

covalent linkagemassively parallel assayprotease substrate specificityProtein displayprotein-protein interactionprotein solubility

Identifiers

PMID41040332
PMCPMC12485679

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.