Evidence map›Paper›PMID 41040199›Full record

ArticlebioRxiv : the preprint server for biology2025

Cytoplasmic localization of PUS7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.

Minli Ruan, Sean M Engels, Matthew R Burroughs, Xiaoyan Li, Rosella Stower, Talia Tzadikario, Connor Powell, Dylan Bloch, Oleksandra Fanari, Stuart Akeson and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Minli RuanUniversity of Michigan, Department of Biological Chemistry, Ann Arbor, MI 48109.
Sean M EngelsUniversity of Texas, McKetta Department of Chemical Engineering, Austin, TX 78712.
Matthew R BurroughsUniversity of Texas, McKetta Department of Chemical Engineering, Austin, TX 78712.ORCID 0000-0002-4866-6050
Xiaoyan LiUniversity of Michigan, Department of Chemistry, Ann Arbor, MI 48109.
Rosella StowerUniversity of Michigan, Department of Biological Chemistry, Ann Arbor, MI 48109.
Talia TzadikarioNortheastern University, Department of Bioengineering, Boston, MA 02120.
Connor PowellNortheastern University, Department of Bioengineering, Boston, MA 02120.
Dylan BlochNortheastern University, Department of Bioengineering, Boston, MA 02120.
Oleksandra FanariNortheastern University, Department of Bioengineering, Boston, MA 02120.
Stuart AkesonNortheastern University, Department of Bioengineering, Boston, MA 02120.
Daniel E EylerUniversity of Michigan, Department of Chemistry, Ann Arbor, MI 48109.
Chase A WeidmannUniversity of Michigan, Department of Biological Chemistry, Ann Arbor, MI 48109.ORCID 0000-0002-2941-0500
Sara RouhanifardNortheastern University, Department of Bioengineering, Boston, MA 02120.ORCID 0000-0002-6991-4877
Miten JainNortheastern University, Department of Bioengineering, Boston, MA 02120.ORCID 0000-0002-4571-3982
Lydia M ContrerasUniversity of Texas, McKetta Department of Chemical Engineering, Austin, TX 78712.
Kristin S KoutmouUniversity of Michigan, Department of Biological Chemistry, Ann Arbor, MI 48109.ORCID 0000-0002-7763-9262

Funding

Synthetic mRNA Control Set for Nanopore-Based Pseudouridine Modification Profiling in Human TranscriptomesR01HG012856 · NHGRI · NORTHEASTERN UNIVERSITY · PI Sara Hakim Rouhanifard · 2023 to 2026
$3.4M
Mechanisms of translational controlR35GM128836 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Kristin S Koutmou · 2018 to 2026
$3.2M
Single-cell direct RNA sequencing using electrical zero-mode waveguides and engineered reverse transcriptasesR01HG011087 · NHGRI · NORTHEASTERN UNIVERSITY · PI WANUNU, MENI · 2020 to 2023
$3.0M
Unraveling the MALAT1 lncRNA-protein interaction networks that drive lung cancer metastasisK22CA262349 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WEIDMANN, CHASE A · 2021 to 2023
$486k
Air pollution-induced Regulation of m6A methylations via ADAR oxidationR21ES032124 · NIEHS · UNIVERSITY OF TEXAS AT AUSTIN · PI CONTRERAS, LYDIA MARIA · 2020 to 2021
$426k
NCI NIH HHS K22 CA262349NHGRI NIH HHS R01 HG011087NHGRI NIH HHS R01 HG012856NIEHS NIH HHS R21 ES032124NIGMS NIH HHS R35 GM128836
6 · The paper itself

Abstract

Pseudouridine (Ψ) is an abundant post-transcriptional modification found across all classes of RNA. It has been widely speculated that Ψ inclusion in mRNAs might provide an avenue for cells to control gene expression post-transcriptionally. Here we demonstrate that one of the principal mRNA pseudouridylating enzymes, pseudouridine synthase 7 (PUS7), exhibits a stress-induced accumulation in the cytoplasm of yeast and human epithelial lung cells. Stress-induced and cytoplasmic localization of PUS7 promote Ψ-incorporation into hundreds of mRNA targets. Furthermore, engineered PUS7 cytoplasmic localization increases cellular fitness under ROS and divalent metal ion stress. Consistent with this, transcripts modified upon PUS7 cytoplasmic localization are enriched within mRNAs encoding proteins involved in divalent metal metabolism and ROS stress pathways. In contrast, tRNA sites modified by PUS7 (Ψ13 and Ψ35 are unperturbed). Quantitative proteomics reveal a reshaping of the proteome upon PUS7 relocalization under stress, with proteins involved in metal and ROS homeostasis being particularly sensitive to PUS7 localization. Collectively, our data demonstrate that PUS7 localization alters mRNA pseudouridylation patterns to modulate protein production and enhance cellular fitness.

Identifiers

PMID41040199
PMCPMC12485686

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.