Evidence map›Paper›PMID 41040161›Full record

ArticlebioRxiv : the preprint server for biology2025

RHO1-2 meganuclease gene editing targets human P23H rhodopsin-induced retinitis pigmentosa to rejuvenate rods and maintain cones.

Archana Jalligampala, Jacob M Young, Jack Feist, Wei Wang, Francesca Barone, David C Alston, James W Fransen, Gita Jaikumar, Kautuk Kamboj, Caitlin Mooreman and 21 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors.

Archana JalligampalaDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Jacob M YoungDepartment of Biochemistry and Molecular Genetics, University of Louisville, Louisville, KY 40202, USA.
Jack FeistDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Wei WangDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Francesca BaroneOcular and Stem Cell Translational Research Section, National Eye Institute, NIH, Bethesda, MD, 20892, USA.
David C AlstonDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
James W FransenDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Gita JaikumarDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Kautuk KambojDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Caitlin MooremanDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Stephen NashDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Jennifer M NoelDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Gobinda PangeniDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Joseph C PrestigiacomoDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Bhubanananda SahuDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Caitlin TurnerPrecision BioSciences, Durham, NC, 27701, USA.
Henry J KaplanDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
Jonathan A GreenDivision of Animal Sciences, Swine Somatic Cell Genome Editing Center, University of Missouri, Columbia, MO, 65211, USA.
Kevin D WellsDivision of Animal Sciences, Swine Somatic Cell Genome Editing Center, University of Missouri, Columbia, MO, 65211, USA.
Victor V BartsevichPrecision BioSciences, Durham, NC, 27701, USA.
Jon E ChattertonPrecision BioSciences, Durham, NC, 27701, USA.
Mara DavisPrecision BioSciences, Durham, NC, 27701, USA.
Kathryn S EvansPrecision BioSciences, Durham, NC, 27701, USA.
Janel LapePrecision BioSciences, Durham, NC, 27701, USA.
Whitney C LewisPrecision BioSciences, Durham, NC, 27701, USA.
Rebecca van de BeekPrecision BioSciences, Durham, NC, 27701, USA.
Kristi D VilesPrecision BioSciences, Durham, NC, 27701, USA.
Derek JantzPrecision BioSciences, Durham, NC, 27701, USA.
Ronald G GreggDepartment of Biochemistry and Molecular Genetics, University of Louisville, Louisville, KY 40202, USA.
Jeff SmithPrecision BioSciences, Durham, NC, 27701, USA.
Maureen A McCallDepartment of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, Louisville, KY, 40202, USA.

Funding

Resource Core for National Swine Resource and Research Center (NSRRC) Years 21-25U42OD011140 · OD · UNIVERSITY OF MISSOURI-COLUMBIA · PI Kiho Lee, Kevin Dale Wells · 2012 to 2026
$26.7M
Swine Somatic Cell Genome Editing (SCGE) CenterU42OD027090 · OD · UNIVERSITY OF MISSOURI-COLUMBIA · PI SAFRANSKI, TIMOTHY J · 2019 to 2023
$9.3M
Summer Vision Sciences Training ProgramT35EY026509 · NEI · UNIVERSITY OF LOUISVILLE · PI BRIAN P. CERESA, Maureen A McCall · 2017 to 2026
$416k
NEI NIH HHS T35 EY026509NIH HHS U42 OD011140NIH HHS U42 OD027090
6 · The paper itself

Abstract

Autosomal dominant retinitis pigmentosa (adRP) is an inherited retinal dystrophy characterized by progressive vision loss and eventual blindness. The P23H mutation (proline to histidine substitution at codon 23) in the rhodopsin (RHO) gene represents the most common form of adRP in North Americans. Currently, there is no cure for P23H adRP. Genome editing targeting the mutant RHO allele, leaving a functional wildtype (WT) allele, is an attractive approach for P23H adRP, as only one copy of RHO is needed for normal retinal function. We re-engineered an I-Cre meganuclease, called RHO1-2, to target a 22bp recognition sequence encompassing the mutation responsible for the p.P23H RHO mutation.

Identifiers

PMID41040161
PMCPMC12485717

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.