ArticleBMC microbiology2025
Streptococcus lishijunsis sp. nov. isolated from feces of Apodemus Chevrieri in western china and its genome function analysis.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
backgroundTwo gram-positive, non-motile, and α-hemolytic streptococci strains, zg-JUN1979T and zg-JUN1980, were isolated from the feces of Apodemus chevrieri in Weining County, Guizhou Province, China. This study aimed to characterize these strains through polyphasic taxonomy and genomic taxonomy, determine their taxonomic classification within the genus Streptococcus.
methodsThe strains were analyzed using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), 16 S rRNA gene sequencing, whole-genome sequencing (Illumina NovaSeq X Plus), average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH). Phenotypic traits were assessed via Biolog GEN III, API 50CH, and API ZYM assays, while chemotaxonomic analysis included fatty acid profiling, polar lipid characterization, and respiratory quinone detection. Genomic functional potential was evaluated using the Comprehensive Antibiotic Resistance Database (CARD), Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), Non-Redundant protein (NR), Pathogen-Host Interaction Database (PHI), and Virulence Factor Database (VFDB).
resultsPhylogenetic analysis based on 16 S rRNA gene sequences revealed 97.35% similarity to Streptococcus hyointestinalis NCTC 12,224T, with distinct clade separation. Whole-genome sequencing confirmed analysed strains as conspecific (ANI = 98.99%, dDDH = 90.6%), but both diverged significantly from S. hyointestinalis (ANI = 72.01%, dDDH = 20.6%). Phenotypically, analysed strains uniquely utilized gentiobiose, sucrose, and α-ketoglutaric acid. Chemotaxonomic analysis identified predominant fatty acids C14:0 (30.8%) and C16:0 (34.1%), a polar lipid profile including diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and phosphatidylcholine (PC), and absence of respiratory quinones. The genome G + C content was 40.06 mol%. Genomic analysis revealed reduced virulence factors (e.g., defective urease activity) alongside antibiotic resistance genes, suggesting a mixed commensal-pathogenic profile.
conclusionBased on molecular, phenotypic, and chemotaxonomic evidence, we propose Streptococcus lishijunsis sp. nov. (zg-JUN1979T = CGMCC 1.65027T = GDMCC 1.5474T = JCM 3776T) as a novel species. Its genomic features indicate adaptation for colonization rather than invasive infection, highlighting ecological and potential clinical implications for wildlife-associated streptococci.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.