Evidence map›Paper›PMID 41039126›Full record

ArticleWorld journal of microbiology & biotechnology2025

Klebsiella aerogenes PgaB orthologue can efficiently hydrolyze Staphylococcus aureus biofilms.

Jéssica Pinheiro Silva, Andrei Nicoli Gebieluca Dabul, Vera Lúcia Mores Rall, Caroline Rosa Silva, Luís Antônio Esmerino, Marcos Pileggi, Felipe Francisco Tuon, Mario de Oliveira Neto, Darlan Nakayama, Alejandra Estela Miranda and 2 more

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jéssica Pinheiro SilvaSão Carlos Institute of Physics, University of São Paulo, Avenida Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil.
Andrei Nicoli Gebieluca DabulSão Carlos Institute of Physics, University of São Paulo, Avenida Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil.
Vera Lúcia Mores RallInstitute of Biosciences, Sao Paulo State University, District of Rubiao Jr, Botucatu, SP, 18618‑970, Brazil.
Caroline Rosa SilvaEnvironmental Microbiology Laboratory, Life Sciences and Health Institute, Structural and Molecular Biology, and Genetics Department, Ponta Grossa State University, Ponta Grossa, Brazil.
Luís Antônio EsmerinoEnvironmental Microbiology Laboratory, Life Sciences and Health Institute, Structural and Molecular Biology, and Genetics Department, Ponta Grossa State University, Ponta Grossa, Brazil.
Marcos PileggiEnvironmental Microbiology Laboratory, Life Sciences and Health Institute, Structural and Molecular Biology, and Genetics Department, Ponta Grossa State University, Ponta Grossa, Brazil.
Felipe Francisco TuonDepartment of Medicine, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.
Mario de Oliveira NetoInstitute of Biosciences, Sao Paulo State University, District of Rubiao Jr, Botucatu, SP, 18618‑970, Brazil.
Darlan NakayamaSão Carlos Institute of Physics, University of São Paulo, Avenida Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil.
Alejandra Estela MirandaSão Carlos Institute of Physics, University of São Paulo, Avenida Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil.
Pedro Ricardo Vieira HamannSão Carlos Institute of Physics, University of São Paulo, Avenida Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil.
Igor PolikarpovSão Carlos Institute of Physics, University of São Paulo, Avenida Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil. ipolikarpov@ifsc.usp.br.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 306852/2021-7Conselho Nacional de Desenvolvimento Científico e Tecnológico 383307/2024-4Fundação de Amparo à Pesquisa do Estado de São Paulo 2021/08780-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/07897-8
6 · The paper itself

Abstract

Staphylococcus aureus, a gram-positive bacterium, is the prevalent cause of numerous infections. Its ability to form biofilms significantly enhances its pathogenicity, resulting in increased antibiotic resistance and evasion of the host immune response. Poly-β-(1,6)-N-acetylglucosamine (PNAG) plays a crucial role in the formation and maintenance of S. aureus biofilms. In this study, we heterologously expressed KaPgaB from Klebsiella aerogenes and evaluated its efficacy in both degrading and inhibiting S. aureus biofilm formation. Additionally, we investigated the combined effects of KaPgaB with DNase I and papain. Our results demonstrated that KaPgaB alone removed up to 81% of biofilm biomass within 4 h when used at a concentration of 0.5 mg/mL. Moreover, when the enzyme was applied sequentially with DNase I, approximately 97% of adhered biofilms were removed. We also observed significant inhibition of biofilm formation across S. aureus strains. The findings presented in this study might be useful for the development of enzymatic tools capable of degrading S. aureus PNAG-based biofilms.

Indexed as

Bacterial ProteinsBiofilmsEnterobacter aerogenesStaphylococcus aureusAcetylglucosamineAnti-Bacterial Agentsbeta-GlucansDeoxyribonuclease IHydrolysisAcetylglucosamineAnti-Bacterial AgentsBacterial Proteinsbeta-GlucansDeoxyribonuclease Ipoly-N-acetyl-1-6-glucosamineBiofilm degradation, KaPgaBPNAGStaphylococcus aureus

Identifiers

PMID41039126

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.