Evidence map›Paper›PMID 41039014›Full record

ArticleScientific reports2025

Normothermic machine perfusion converges transplant induced transcript profiles in low and high risk DBD and DCD livers.

Karen S Keslar, Qiang Liu, William M Baldwin, Brian T Gaudette, Chase J Wehrle, Keyue Sun, Chunbao Jiao, Mingyi Zhang, Khaled Ali, Beatrice Cazzaniga and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Advancements inFrontiers in transplantation · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Karen S Keslar *Department of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, NB3-59, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
Qiang Liu *Digestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH, USA.
William M BaldwinDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, NB3-59, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
Brian T GaudetteDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, NB3-59, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
Chase J WehrleDigestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH, USA.
Keyue SunDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, NB3-59, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
Chunbao JiaoDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, NB3-59, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
Mingyi ZhangDigestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH, USA.
Khaled AliDigestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH, USA.
Beatrice CazzanigaDigestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH, USA.
Luca Del PreteDigestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH, USA.
Charles MillerDigestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH, USA.
Koji HashimotoDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, NB3-59, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
Andrea SchlegelDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, NB3-59, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
Cristiano QuintiniDigestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH, USA. quintic2@clevelandclinicabudhabi.ae.
Robert L FairchildDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, NB3-59, 9500 Euclid Avenue, Cleveland, OH, 44195, USA. fairchr@ccf.org.

Funding

POSTTRANSPLANT INFLAMMATORY RESPONSER01AI040459 · NIAID · CLEVELAND CLINIC LERNER COM-CWRU · PI Robert L Fairchild · 1997 to 2026
$9.4M
National Institute of Allergy and Infectious Diseases RO1-AI40459NIAID NIH HHS R01 AI040459
6 · The paper itself

Abstract

Normothermic machine perfusion (NMP) of donated livers is emerging as a superior preservation technology versus static cold storage (SCS) for transplant, transforming a metabolically dormant organ to a functioning state that improves outcomes of marginal liver transplants. Mechanisms underlying improved NMP restoration of donation after brain death (DBD) and donation after circulatory death (DCD) livers remain incompletely defined. We tested the hypothesis that NMP would decrease transcripts encoding inflammatory mediators following both perfusion and transplant of DBD and DCD livers. Biopsy transcript expression profiles were determined from SCS DBD livers at pre-SCS preservation and transplant reperfusion and from benchmark and outside benchmark criteria DBD and DCD livers pre-NMP, after 6-7 h of NMP, and after transplant reperfusion. Compared to SCS livers, NMP programs converged transcript profiles of DCD and DBD livers, decreasing transplant reperfusion-induced inflammatory transcripts while increasing transcripts promoting wound healing and tissue repair processes, but did not obviate the high occurrence of adverse events in the outside benchmark DCD livers transplants.

Indexed as

Brain DeathLiverLiver TransplantationOrgan PreservationPerfusionTranscriptomeAdultFemaleGene Expression ProfilingHumansMaleMiddle AgedTissue DonorsGene expressionGraft preservationInflammationIschemia-reperfusion injuryLiver transplantation

Identifiers

PMID41039014
PMCPMC12491471

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.