ArticleScientific reports2025
Biofilm production and virulence traits among extensively drug-resistant and methicillin-resistant Staphylococcus aureus from buffalo subclinical mastitis in Bangladesh.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Molecular Prevalence and Antimicrobial Resistance Patterns of Methicillin-Resistant Staphylococcus aureus in Buffaloes from Bangladesh.Veterinary medicine and science · 2026Article
- Genetic Diversity and Epidemiological Overlap of Staphylococcus aureus at the Animal-Food-Environment-Human Interface Within a One Health Framework.MicrobiologyOpen · 2026Article
- Predominance of SCCmec type III, multidrug resistance, and evidence of ica-independent biofilm formation among clinical Staphylococcus aureus isolates in Riyadh, Saudi Arabia.BMC infectious diseases · 2026Article
- Review
- Antimicrobial resistance profiles of non-aureus Staphylococci isolated from farm animals, farm environments and companion animals.Scientific reports · 2026Article
- Microbial Distribution and Biofilm-Forming Capacity in the Reproductive Tract of Farm Ruminants.Animals : an open access journal from MDPI · 2026Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a critical pathogen implicated in subclinical mastitis (SCM), a hidden threat to dairy productivity. This study investigated the prevalence, antibiotic resistance profiles, and virulence traits of MRSA from SCM-affected riverine buffaloes in Jamalpur, Bangladesh. A total of 344 milk samples were screened using the California Mastitis Test (CMT) and Modified Whiteside Test (MWST). Among the milk samples, 46.5% were positive for SCM by CMT. Culture, biochemical tests, and PCR confirmed 73 (21.2%) Staphylococcus spp., of which 30 (41.1%) were identified as S. aureus and 43 (58.9%) as non-aureus staphylococci (NAS). Among the 30 S. aureus-positive isolates, 10 (33.3%) were identified as methicillin-resistant S. aureus (MRSA), corresponding to a prevalence of 2.9% among the total milk samples. The MRSA isolates exhibited high multidrug resistance, especially to tetracycline (80%) and cefoxitin (80%), and commonly harbored resistance genes such as tetA (80%), aac(3)-iv (70%), and sul1 (50%). Virulence genes hla (66.7%) and sea (50%) were frequently detected, while icaA was found in 23.3% of MRSA. Notably, 60% of MRSA isolates were categorized as XDR based on international standard definitions, while 60% were biofilm producers with high MARI values up to 0.92, indicating severe resistance potential. These findings underscore a significant burden of MDR/XDR MRSA with virulence potential in buffalo SCM, posing serious risks to animal and public health.
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