Evidence map›Paper›PMID 41038875›Full record

ArticleScientific reports2025

DNAJC5 promotes cisplatin resistance in epithelial ovarian cancer by autophagy induced by the BiP/IRE1α/XBP1 endoplasmic reticulum stress pathway.

Shijia Huang, Ling Chen, Yirong Chen, Siyan Lu, Bowen Yang, Ya Chen, Wei Zhao, Shuli Zhao

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Shijia Huang *General Clinical Research Center, Nanjing First Hospital, China Pharmaceutical University, Nanjing, Jiangsu, China.
Ling Chen *Department of Pathology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu, China.
Yirong ChenGeneral Clinical Research Center, Nanjing First Hospital, China Pharmaceutical University, Nanjing, Jiangsu, China.
Siyan LuGeneral Clinical Research Center, Nanjing First Hospital, China Pharmaceutical University, Nanjing, Jiangsu, China.
Bowen YangGeneral Clinical Research Center, Nanjing First Hospital, China Pharmaceutical University, Nanjing, Jiangsu, China.
Ya ChenDepartment of Pathology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu, China.
Wei ZhaoDepartment of Pathology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
Shuli ZhaoGeneral Clinical Research Center, Nanjing First Hospital, China Pharmaceutical University, Nanjing, Jiangsu, China. shulizhao79@njmu.edu.cn.

Funding

National Natural Science Foundation of China 82173205
6 · The paper itself

Abstract

Ovarian cancer is the most lethal gynecological malignancy, with platinum-based chemotherapy serving as the standard first-line treatment. However, the emergence of primary or acquired resistance significantly compromises patient survival. Among the mechanisms contributing to chemotherapy resistance, autophagy plays a crucial role. DnaJ heat shock protein family (Hsp40) member C5 (DNAJC5) is a key regulator of nervous system function. Recent studies suggest that DNAJC5 may also be involved in tumor progression. Based on this, we aimed to investigate the role of DNAJC5 in epithelial ovarian cancer (EOC) chemotherapy resistance. Survival analysis of the TCGA database revealed a strong association between elevated DNAJC5 expression and poor prognosis in ovarian cancer patients. Immunohistochemistry (IHC) and statistical analyses demonstrated that patients with high DNAJC5 expression frequently presented with advanced International Federation of Gynecology and Obstetrics (FIGO) stage and elevated CA125 levels. Functional experiments showed that overexpression of DNAJC5 in A2780 cells markedly increased resistance to cisplatin (DDP). Moreover, cisplatin-resistant EOC cell lines (A2780/DDP) exhibited high levels of DNAJC5 protein expression. Conversely, DNAJC5 knockdown enhanced cisplatin-induced apoptosis and inhibited autophagy in A2780/DDP cells, as confirmed by Annexin V/PI staining, CCK-8 assays, colony formation assays, and Western blot analysis. Further mechanistic investigations using RNA sequencing identified a strong correlation between DNAJC5 expression and endoplasmic reticulum (ER) stress. Western blot analysis confirmed that DNAJC5 overexpression upregulated BiP-IRE1α-XBP1 protein expression, which was significantly associated with both increased autophagy levels and enhanced DDP resistance. These findings were further supported by validation experiments using hydroxychloroquine(CQ) and 4µ8C, as well as in vivo animal studies. In conclusion, this study demonstrates that DNAJC5 overexpression promotes DDP resistance in EOC by modulating autophagy through the BiP-IRE1α-XBP1 signaling pathway.

Indexed as

AutophagyCarcinoma, Ovarian EpithelialCisplatinDrug Resistance, NeoplasmEndoplasmic Reticulum StressHSP40 Heat-Shock ProteinsOvarian NeoplasmsX-Box Binding Protein 1AnimalsAntineoplastic AgentsCell Line, TumorEndoplasmic Reticulum Chaperone BiPEndoribonucleasesFemaleGene Expression Regulation, NeoplasticHeat-Shock ProteinsAntineoplastic AgentsCisplatinEndoplasmic Reticulum Chaperone BiPEndoribonucleasesERN1 protein, humanHeat-Shock ProteinsHSP40 Heat-Shock ProteinsProtein Serine-Threonine KinasesX-Box Binding Protein 1XBP1 protein, humanAutophagyCisplatin resistanceDNAJC5Endoplasmic reticulum stressEpithelial ovarian cancer

Identifiers

PMID41038875
PMCPMC12491438

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.