Evidence map›Paper›PMID 41036618›Full record

ArticleNucleic acids research2025

WDR5 serves in co-activation and influences genome targeting of KLF3.

Lu Yang, Manan Shah, Tanit Chavalit, Annalise M Spek, Wooi F Lim, Ling Zhong, Vala Nejad Safari, Mahdi Haddad, Thu H Vu, Joel P Mackay and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Lu YangSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.
Manan ShahSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.ORCID 0000-0003-1390-5450
Tanit ChavalitSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.
Annalise M SpekSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.
Wooi F LimSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.
Ling ZhongBioanalytical Mass Spectrometry Facility, UNSW Sydney, Sydney, NSW 2052, Australia.
Vala Nejad SafariSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.
Mahdi HaddadSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.
Thu H VuSchool of Life and Environmental Sciences, University of Sydney, NSW 2006, Australia.
Joel P MackaySchool of Life and Environmental Sciences, University of Sydney, NSW 2006, Australia.ORCID 0000-0001-7508-8033
Mark J RafteryBioanalytical Mass Spectrometry Facility, UNSW Sydney, Sydney, NSW 2052, Australia.
Marc R WilkinsSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.
Jacqueline M MathewsSchool of Life and Environmental Sciences, University of Sydney, NSW 2006, Australia.
Kate G R QuinlanSchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.ORCID 0000-0002-5722-8183
Merlin CrossleySchool of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.ORCID 0000-0003-2057-3642

Funding

Australian Research Council DP170101786Australian Research Council DP220101489Australian Research Council DP250103666China Scholarship CouncilNational Collaborative Research Infrastructure Strategy (NCRIS)Research Training ProgramRoyal Thai Government scholarshipUNSW Sydney University
6 · The paper itself

Abstract

Krüppel-like factor 3 (KLF3) is a member of the archetypal SP/KLF family of transcription factors that bind GC-rich elements and CACCC boxes in promoters and enhancers via three classical zinc fingers (ZFs) at or near their C-termini. KLF3 can both repress and activate transcription. It represses by recruiting CtBP co-repressors via its N-terminal domain but the mechanism by which it activates was unknown. Here, we show that KLF3 associates with WDR5 and this interaction is associated with gene activation. We also demonstrate that this interaction is required for proper genomic targeting of KLF3. This helps explain previous results indicating that both the C-terminal ZF DNA-binding domain and N-terminal functional domain are required for KLF3 to identify its target genes. This result adds to only a handful of examples that transcriptional co-regulators, in addition to facilitating activation and repression, can also influence target gene selection.

Indexed as

Histone-Lysine N-MethyltransferaseKruppel-Like Transcription FactorsTranscriptional ActivationHEK293 CellsHumansIntracellular Signaling Peptides and ProteinsPromoter Regions, GeneticProtein BindingZinc FingersHistone-Lysine N-MethyltransferaseIntracellular Signaling Peptides and ProteinsKLF3 protein, humanKruppel-Like Transcription FactorsWDR5 protein, human

Identifiers

PMID41036618
PMCPMC12481014

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.