ArticleOpen veterinary journal2025
Morphometrics analysis and molecular identification of the
Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Molecular Survey ofVeterinary sciences · 2026Article
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Authors and funding
8 authors.
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Abstract
Background: Morphologically, the species within the subgenus Trypanozoon cannot be clearly distinguished. Aim: To identify and characterize trypanosome species in Indonesia through a molecular algorithm that was carried out in stages using four pairs of primers to confirm and distinguish between species of the subgenus Methods: A total of 28 trypanosome isolates were examined microscopically, and their morphobiometrics were observed. The isolates were also identified molecularly with an identification algorithm involving four pairs of primers in a hierarchical manner, namely, ITS1, ESAG6/7, minicircle, and maxicircle. Results: Morphologically, Indonesian trypanosomes are characterized by a long, slender shape, small kinetoplasts located below the posterior terminal end, and free flagella with conspicuous undulating membranes. Screening using ITS1 and ESAG6/7 showed a single band at 480 and 237 bp, which indicates the subgenus Conclusions: Molecular identification algorithms can distinguish species of the subgenus Clinical Relevance: The minicircle and maxicircle primers are the key to species identification of Trapanozoon which can be applied to overcome the identification difficulties that have been experienced so far.
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