ArticleOpen veterinary journal2025
Effects of trehalose supplementation on Pesisir bull sperm quality during storage.
Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Semen preservation is a critical component of artificial insemination (AI) in livestock breeding, with storage conditions playing a vital role in maintaining sperm quality. Pesisir bulls, an indigenous breed from West Sumatra, Indonesia, face reproductive challenges due to limited breeding programs and suboptimal semen preservation techniques. Trehalose, a non-reducing disaccharide, is recognized for its cryoprotective and antioxidant properties, which help preserve sperm motility, viability, and membrane integrity during storage. However, the optimal concentration of trehalose for liquid semen storage in Pesisir bulls remains undetermined. Aim: This study evaluated the effects of trehalose supplementation in a Tris-egg yolk extender on the motility, viability, plasma membrane integrity, and acrosome integrity of Pesisir bull sperm stored at 5°C for 72 hours. Methods: Ejaculates from Pesisir bulls with ≥80% individual motility were collected weekly using an artificial vagina. Semen was diluted with a Tris-egg yolk extender containing 0, 15, 30, or 45 mM trehalose to achieve a final concentration of 25 million spermatozoa per ml. Sperm quality parameters were assessed at 0, 24, 48, and 72 hours post-dilution. Results: Fresh semen had a volume of 3.5 ± 0.2 ml, a milky white color, thick consistency, a pH of 6.8 ± 0.1, and a characteristic seminal odor. Microscopically, it exhibited +++ mass movement, a concentration of 1,465 ± 50 × 10 Conclusion: Trehalose supplementation in a Tris-egg yolk extender enhanced the preservation of Pesisir bull semen during liquid storage. A concentration of 30 mM trehalose was found to be optimal for maintaining sperm quality at 5°C, offering potential benefits for artificial insemination programs and genetic conservation initiatives.
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