ArticleOpen veterinary journal2025
Reduction of post-thawed sperm quality of Kacang goat using the addition of epigallocatechin-3-gallate into the skim milk-egg yolk extender.
Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: One native goat species in Indonesia that faces extinction because of cross-breeding is the Kacang goat ( Aim: The purpose of this study was to investigate the effect of the addition of epigallocatechin-3-gallate nanoparticles (EGCG) to skimmed egg yolk (SM-EY) extender on the quality of post-thawed Kacang buck spermatozoa for artificial insemination. Methods: Fresh semen of Kacang buck was diluted in SM-EY without (T0) and with the addition of 50 or 100 mg EGCG/dl extender for T1 and T2, respectively. Extended semen was frozen in liquid nitrogen (-196°C) for a week in French tiny straws with 60 million spermatozoa per straw, following a standard protocol. Six thawed replicates from each group were tested for sperm motility, viability, intact plasma membrane (IPM), malonaldehyde (MDA), DNA integrity, and chromatin density. Results: Pre-equilibration sperm motility, viability, and IPM were not significantly different among the groups ( Conclusion: The addition of 50 or 100 mg EGCG/dl SM-EY extender significantly improved the post-thawed spermatozoa quality of Kacang buck.
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