Evidence map›Paper›PMID 41034973›Full record

ArticleJournal of ovarian research2025

Identifying ovarian cortex cell subpopulations using multicolor flow cytometry.

Sophie Frontczak, Tristan Zver, Jean-Baptiste Pretalli, Oxana Blagosklonov, Clotilde Amiot, Christophe Roux, Frederic Grenouillet, Florence Scheffler

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In one paragraph

Article in Journal of ovarian research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sophie FrontczakUniversité Marie et Louis Pasteur, CHU Besançon, Besançon, F-25000, France. s1franck@chu-besancon.fr.
Tristan ZverCHU Besançon, Service de Biologie et Médecine de la Reproduction, Cryobiologie, CECOS Bourgogne Franche-Comté, Besançon, F-25000, France.
Jean-Baptiste PretalliINSERM CIC-1431, CHU Besançon, Besançon, F-25000, France.
Oxana BlagosklonovUniversité Marie et Louis Pasteur, CHU Besançon, Besançon, F-25000, France.
Clotilde AmiotUniversité Marie et Louis Pasteur, CHU Besançon, Besançon, F-25000, France.
Christophe RouxUniversité Marie et Louis Pasteur, CHU Besançon, Besançon, F-25000, France.
Frederic GrenouilletUniversité Marie et Louis Pasteur, CHU Besançon, Besançon, F-25000, France.
Florence SchefflerService de Gynécologie-Obstétrique, CHU Besançon, Besançon, F-25000, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

contextOvarian tissue autotransplantation is currently the only proven technique for reusing ovarian tissue after fertility preservation by ovarian tissue cryopreservation. However, one of its limitations relates to the quality of the grafts, both in terms of the number of surviving follicles and the quality of the stromal environment, which is essential for follicular development and graft revascularization. The aim of this study was to validate a technique for characterizing and functionally qualifying ovarian tissue in order to identify cell sub-populations of interest. MATERIALS: Ovarian cortex strips were collected during ovarian drilling in women suffering from polycystic ovary syndrome. After fresh or frozen ovarian tissue dissociation, the resulting ovarian cells were analyzed by multicolor flow cytometry (MFC) to determine cell yield and viability after dissociation, and to identify for specific with specific antibodies.

resultsYield was significantly higher after dissociation of fresh ovarian tissue (1,59 × 10

conclusionAlthough further panel development is required, MFC effectively characterizes cell populations within ovarian tissue. Non-follicular cells could be evaluated as a potential prognostic factor for the recovery of ovarian function after autotransplantation but also participate in ovarian reconstruction programs.

Indexed as

Flow CytometryOvaryAdultCell SurvivalCryopreservationFemaleFertility PreservationHumansFertility preservationMulticolor flow cytometryOvarian tissueOvarian tissue cryopreservationQualificationStromal cell

Identifiers

PMID41034973
PMCPMC12487478

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