ArticleBMC plant biology2025
Metabolome and transcriptome based investigation of anthocyanin accumulation in black wolfberry (Lycium ruthenicum) and validation of the function of LrUFGT.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundBlack wolfberry (Lycium ruthenicum Murray) is renowned for its abundant anthocyanins, exhibiting antioxidant, anti-inflammatory, and anti-ageing activities. However, the accumulation patterns and molecular regulation of anthocyanins during fruit maturation are poorly understood. This study examined anthocyanin biosynthesis at three different stages: young fruit (LR-1), color transition (LR-2), and mature fruit (LR-3).
resultsThe analysis revealed higher levels of anthocyanins in LR-3 (12.83 mg/g) than in LR-2 (9.76 mg/g) and LR-1 (8.41 mg/g), which established a correlation between anthocyanin accumulation and pericarp pigmentation. Antioxidant capacity assays demonstrated an increase in total antioxidant activity and hydroxyl radical scavenging ability during maturation. HPLC profiling identified three anthocyanidin aglycones: cyanidin, pelargonidin and delphinidin. LR-3 exhibited predominant cyanidin accumulation, while LR-1 lacked pelargonidin. Furthermore, the metabolomic analysis revealed that, the accumulation of three specific anthocyanins cyanidin-3,5-di-O-glucoside, cyanidin-3-sophoroside-5-glucoside, and pelargonidin, serves as the primary determinant of color variation in black wolfberry fruits. A transcriptomic investigation revealed the activation of structural genes (LrCHI, LrF3'H, LrF3'5'H) and glycosyltransferase gene LrUFGT. WGCNA and correlation analyses were then performed to identify networks coordinating anthocyanin biosynthesis. Functional characterization confirmed that chloroplast-localized LrUFGT catalysis cyanidin glycosylation to produce cyanidin-3-O-glucoside. Heterologous expression of LrUFGT in yeast led to enhanced tolerance to salinity and drought stress.
conclusionsThis multi-omics study provides a mechanistic understanding of anthocyanin regulation in black wolfberry, identifying targets for developing novel germplasms with elevated anthocyanin content. These findings establish a foundation for breeding strategies to enhance the nutritional quality and commercial value of black wolfberry.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.