Evidence map›Paper›PMID 41034431›Full record

Trial reportEuropean journal of applied physiology2026

The effects of aerobic and resistance exercise on the lipid profile of extracellular vesicles.

Tesha Kerr Carpenter, Mariah McCashland, Raechel Sherrick, Sathish Kumar Natarajan, Mirko Mandić, Stephen D Kachman, Ferdinand von Walden, Ivan J Vechetti, Rodrigo Fernandez-Gonzalo

Abstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in European journal of applied physiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tesha Kerr CarpenterDepartment of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Mariah McCashlandDepartment of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Raechel SherrickDepartment of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Sathish Kumar NatarajanDepartment of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Mirko MandićDepartment of Laboratory Medicine, Division of Clinical Physiology, Karolinska Institutet, Stockholm, Sweden.
Stephen D KachmanDepartment of Statistics, University of Nebraska-Lincoln, Lincoln, NE, USA.
Ferdinand von WaldenDepartment of Women's and Children's Health, Karolinska Institutet, Solna, Sweden.
Ivan J VechettiDepartment of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA. ivechetti@unl.edu.
Rodrigo Fernandez-GonzaloDepartment of Laboratory Medicine, Division of Clinical Physiology, Karolinska Institutet, Stockholm, Sweden. rodrigo.fernandez-gonzalo@ki.se.ORCID http://orcid.org/0000-0003-2816-9855

Funding

AFM-Telethon 23137NIGMS NIH HHS P20GM104320-07Swedish National Space Agency 2021-00159
6 · The paper itself

Abstract

purposeLipids are one of the most abundant molecules within extracellular vesicles (EVs) and are important for EV biology and cell signaling. However, very little attention has been given to the role of lipids in the biological function of EVs during exercise. Therefore, we completed a study using a cross-over design to investigate the effects of aerobic and resistance exercise on the lipid profile of EVs.

methodsTen healthy participants (23.0 ± 3.6 years) performed an acute bout of aerobic exercise, an acute bout of resistance exercise, as well as a period of rest (control) in a randomized, cross-over design. Blood samples were collected immediately following exercise and 30 min after exercise, and exercise conditions were compared to control within each subject. Plasma EVs were isolated using cushioned-density gradient ultracentrifugation (C-DGUC). The EV size, morphology, and protein markers were examined using nanoflow cytometry, transmission electron microscopy, and western blot, respectively. Additionally, we conducted an untargeted lipidomics analysis on the EV isolate.

resultsOur findings revealed neither exercise modality had a significant effect on the size or concentration of EVs (P > 0.05). However, we found that immediately after exercise there was a decrease in glycerophospholipids within the EVs (55% vs 49%; P < 0.05) in the resistance exercise group compared to the control group, a response not found in the aerobic exercise group.

conclusionOur data suggest that although resistance exercise induced some changes in the lipid composition of EVs, the EV-containing lipids do not appear to be a critical mechanism utilized by cells to mediate exercise-induced adaptations.

Indexed as

ExerciseExtracellular VesiclesLipid MetabolismLipidsResistance TrainingAdultCross-Over StudiesFemaleHumansLipidomicsMaleYoung AdultLipidsAerobic exerciseExtracellular vesicleLipidomicsResistance exercise

Identifiers

PMID41034431
PMCPMC13013174

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.