ArticleCommunications biology2025
Deciphering brain organoid heterogeneity by identifying key quality determinants.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Interpretable Multi-Feature Optical Analysis for Stage- and Batch-Aware Quality Assessment of Brain-Organoid Cultures.Sensors (Basel, Switzerland) · 2026Article
- Shape factor analysis as a quantitative framework for assessing spheroid and organoid morphology and invasiveness.APL bioengineering · 2026Article
- Restoring pluripotency to pluripotent stem cells.Nature biotechnology · 2026Article
- Organoids: generation strategies, applications, and future challenges.Stem cell research & therapy · 2026Review
- Shape Factor Analysis as a Quantitative Framework for Assessing Spheroid and Organoid Morphology and Invasiveness.bioRxiv : the preprint server for biology · 2026Article
- Advances in organoids for personalized medicine: from technological development to clinical application.Frontiers in cell and developmental biology · 2026Review
- Generating cerebellar organoids from pluripotent stem cells.Disease models & mechanisms · 2026Review
- Parsing Glomerular and Tubular Structure Variability in High-Throughput Kidney Organoid Culture.Methods and protocols · 2025Article
Corrections and comments
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Authors and funding
20 authors.
Funding
Abstract
Brain organoids derived from human pluripotent stem cells (hPSCs) hold immense potential for modeling neurodevelopmental processes and disorders. However, their experimental variability and undefined organoid selection criteria for analysis hinder reproducibility. As part of the Bavarian ForInter consortium, we generated 72 brain organoids from distinct hPSC lines. We conducted a comprehensive analysis of their morphological and cellular characteristics at an early stage of their development. In our assessment, the Feret diameter emerged as a reliable, single parameter that characterizes brain organoid quality. Transcriptomic analysis of our organoid identified the abundance of unintended mesodermal differentiation as a major confounder of unguided brain organoid differentiation, correlating with Feret diameter. High-quality organoids consistently displayed a lower presence of mesenchymal cells. These findings provide a framework for enhancing brain organoid standardization and reproducibility, underscoring the need for morphological quality controls and considering the influence of mesenchymal cells on organoid-based modeling.
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Registered trials
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