Evidence map›Paper›PMID 41033150›Full record

ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2025

Design and performance of a real-time RT-PCR assay for detection of influenza C viruses.

Bo Shu, William G Davis, Ji Liu, Beth K Thielen, Sarah Bistodeau, Brian Lynch, Christine M Warnes, Jimma Liddell, Anna K Strain, Jaime Christensen and 4 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Bo ShuNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
William G DavisNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
Ji LiuNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
Beth K ThielenDivision of Pediatric Infectious Diseases, University of Minnesota Medical School, Minneapolis, United States.
Sarah BistodeauMinnesota Department of Health, St. Paul, United States.
Brian LynchNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
Christine M WarnesNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
Jimma LiddellNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
Anna K StrainMinnesota Department of Health, St. Paul, United States.
Jaime ChristensenMinnesota Department of Health, St. Paul, United States.
Phili WongNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
Natasha BurnettNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
Todd C DavisNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States.
Marie K KirbyNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, United States. Electronic address: pbi0@cdc.gov.

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

Influenza C virus (ICV) usually causes a mild upper respiratory tract infection in children and those infected are frequently co-infected with other respiratory viruses. However, there have only been a few hundred documented cases of ICV infection in humans as of the end of 2024. To better understand the epidemiology and clinical impact of ICVs, we developed an influenza C real-time RT-PCR (InfC rRT-PCR) assay that targets a highly conserved region of the matrix gene segment of ICVs. The analytical sensitivity evaluation demonstrated that the InfC rRT-PCR assay was highly sensitive, as it was able to detect as few as five RNA copies per PCR reaction and had robust reactivity over a range of viral RNAs from historical and recent ICVs. The analytical specificity evaluation confirmed the assay did not cross-react with any influenza A or B viruses tested, including several animal-origin viruses, or other common non-influenza respiratory viruses. The performance evaluation on clinical specimens demonstrated the assay was highly sensitive and specific for the detection of ICVs.

Indexed as

GammainfluenzavirusInfluenza, HumanMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionChildChild, PreschoolHumansRNA, ViralSensitivity and SpecificityViral Matrix ProteinsRNA, ViralViral Matrix ProteinsDiagnostic assayInfluenza C virusReal-time RT-PCR

Identifiers

PMID41033150
PMCPMC12974223

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.