Evidence map›Paper›PMID 41032137›Full record

ArticleAnalytical and bioanalytical chemistry2025

Next-generation protein sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6.

Kenneth A Skinner, Troy D Fisher, Andrew Lee, Taojunfeng Su, Eleonora Forte, Aniel Sanchez, Michael A Caldwell, Neil L Kelleher

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Kenneth A SkinnerQuantum-Si Incorporated, Branford, CT, USA.ORCID http://orcid.org/0000-0001-6407-5034
Troy D FisherProteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.ORCID http://orcid.org/0009-0006-1306-0397
Andrew LeeDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.ORCID http://orcid.org/0000-0002-7665-1999
Taojunfeng SuDepartment of Molecular Biosciences, Northwestern University, Evanston, IL, USA.ORCID http://orcid.org/0000-0002-7111-1248
Eleonora ForteDivision of Nephrology, Department of Medicine, University of Illinois College of Medicine, Chicago, IL, USA.ORCID http://orcid.org/0000-0002-6095-2797
Aniel SanchezProteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.ORCID http://orcid.org/0000-0002-0278-8802
Michael A CaldwellProteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.ORCID http://orcid.org/0000-0002-8636-0706
Neil L KelleherProteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA. n-kelleher@northwestern.edu.ORCID http://orcid.org/0000-0002-8815-3372

Funding

National Center for Translational and Developmental ProteomicsRM1GM156535 · NIGMS · NORTHWESTERN UNIVERSITY · PI NEIL L KELLEHER · 2025 to 2026
$3.4M
NIGMS NIH HHS RM1 GM156535
6 · The paper itself

Abstract

The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, we evaluated the complementarity of two cutting-edge proteomic technologies-single-molecule protein sequencing and individual ion mass spectrometry-for analyzing recombinant human IL-6 (rhIL-6) at the amino acid, peptide, and intact proteoform levels. For single-molecule protein sequencing, we employed the recently released Platinum instrument. Next-generation protein sequencing (NGPS) on Platinum utilizes cycles of N-terminal amino acid recognizer binding and aminopeptidase cleavage to enable parallelized sequencing of single peptide molecules. We found that NGPS produces single amino acid coverage of multiple key regions of IL-6, including two peptides within helices A and C, which harbor residues that reportedly impact IL-6 function. For top-down proteoform evaluation, we used individual ion mass spectrometry (I

Indexed as

Interleukin-6Mass SpectrometrySequence Analysis, ProteinAmino Acid SequenceHumansProteomicsRecombinant ProteinsIL6 protein, humanInterleukin-6Recombinant ProteinsIndividual ion mass spectrometryNext-generation protein sequencingPlatinumProteoformSingle-molecule protein sequencingTop-down mass spectrometry

Identifiers

PMID41032137
PMCPMC12598678

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.