ArticleMethodsX2025
Establishing a quantitative link between crystal violet absorbance and biomass in biofilms.
Article in MethodsX, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- First Report and Integrated Characterization ofAnimals : an open access journal from MDPI · 2026Article
- In Vitro Biofilm Formation on 3D-Printed, Milled, and Conventionally Manufactured Denture Base Resins.Bioengineering (Basel, Switzerland) · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantifying biomass is essential for studying biofilms in biomanufacturing, yet widely used assays such as crystal violet (CV) often yield results that are difficult to compare across laboratories due to its reliance on absorbance as a subjective proxy for biomass. To address this, we present a standardized method that calibrates CV absorbance against cellular optical density (OD) and dry cell weight (DCW) using centrifuged planktonic cultures. By establishing a three-way correlation among OD, DCW, and CV absorbance, this simple method allows for quantitative, reproducible, and comparable biomass measurements. Validation across
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.