ArticleFrontiers in bioengineering and biotechnology2025
A novel non-invasive sweat lactate biosensor and its application in human exercise monitoring.
Article in Frontiers in bioengineering and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Lactate is a key biomarker for clinical diagnostics and athletic performance monitoring. Conventional blood-based assays are invasive and not ideal for real-time applications. Sweat, as a non-invasive alternative, offers significant advantages for dynamic lactate tracking. Methods: We developed a highly sensitive fluorescence resonance energy transfer (FRET)-based aptasensor for lactate detection in sweat. The sensing platform utilizes aptamer-functionalized core-shell upconversion nanoparticles (APT-CS-UCNPs) as energy donors and Fe Results: The aptasensor exhibited a broad linear detection range (0-30 mM, Discussion: This FRET-based aptasensor enables accurate, rapid, and non-invasive lactate quantification using standard laboratory instrumentation. Its successful application in real human sweat samples highlights strong potential for both clinical diagnostics and athletic performance monitoring.
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