Evidence map›Paper›PMID 41030781›Full record

ArticleBiochemistry and biophysics reports2025

Characterization of adalimumab Fab variants with the CH1 domain replaced by the Cα1 domain of IgA.

Rara Sugimoto, Hitomi Nakamura, Masato Kiyoshi, Akiko Ishii-Watabe, Naoko Oda-Ueda, Takatoshi Ohkuri

Abstract read
In one paragraph

Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Rara SugimotoFaculty of Pharmaceutical Sciences, Sojo University, Japan.
Hitomi NakamuraFaculty of Pharmaceutical Sciences, Sojo University, Japan.
Masato KiyoshiDivision of Biological Chemistry and Biologicals, National Institute of Health Sciences, Japan.
Akiko Ishii-WatabeDivision of Biological Chemistry and Biologicals, National Institute of Health Sciences, Japan.
Naoko Oda-UedaFaculty of Pharmaceutical Sciences, Sojo University, Japan.
Takatoshi OhkuriFaculty of Pharmaceutical Sciences, Sojo University, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antibody constant domains (C domains) contribute to structural stability. However, studies focusing on Fab fragments with heterologous constant domains are limited. Here, we engineered an IgA-type Fab (Fab_CH1IgA) by replacing the CH1 domain of an IgG1-type adalimumab Fab with the corresponding Cα1 domain of IgA1. Fab_CH1IgA was expressed in CHO cells at approximately 50 mg L

Indexed as

AdalimumabFabIgAMutation analysisThermal stability

Identifiers

PMID41030781
PMCPMC12477844

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.