ArticleCureus2025
Targeting Photoaging With Heat-Killed Escherichia coli Nissle 1917: A Novel Cellular Model and Anti-photoaging Strategy.
Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUltraviolet (UV) radiation is a major contributor to skin aging, manifesting as wrinkles, pigmentation, and structural dysfunction, collectively termed photoaging. Chronic photoaging is strongly linked to an increased risk of skin cancer. Despite the growing demand for effective anti-photoaging agents, a gap remains in both understanding the molecular basis of photoaging and validating the efficacy of new therapeutic candidates. This study aimed to establish a reliable in vitro photoaging model and evaluate the potential anti-photoaging effects of heat-killed
methodsHuman dermal fibroblast (HDF) cells were exposed to UVB radiation three times at one-day intervals. Cell viability was assessed using the MTT assay. Post-irradiation, cells were treated with varying concentrations of heat-killed EcN. Effects were evaluated through live/dead staining, apoptosis assay, cell cycle analysis, and reactive oxygen species (ROS) quantification. Resveratrol (RES), a known anti-inflammatory and anti-aging compound, was used as a reference control.
resultsUVB exposure at 0.3 J/cm² reduced HDF cell viability to approximately 50% compared to the control group. Morphological and biochemical assessments validated the photoaging model's reliability. Treatment with 10% v/v heat-killed EcN and 100 μM RES after each UVB cycle significantly restored cell viability, reduced apoptosis and cell cycle arrest, and markedly decreased intracellular ROS levels.
conclusionThis study demonstrates the efficacy of heat-killed EcN and RES in alleviating UVB-induced photoaging, likely via suppression of oxidative stress and apoptosis. The developed in vitro model offers a robust platform for future investigations into molecular mechanisms of photoaging and the evaluation of emerging photoprotective agents.
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