Evidence map›Paper›PMID 41029503›Full record

ArticleBMC cancer2025

Altered expression spectrum and target gene prediction of tRNA-derived small RNAs in clear cell renal cell carcinoma.

Shuai Liu, Heng Cao, Bowen Chen, Lei Jiang, Yimingniyizi Nueraihemaiti, Yuchen Gao, Xiangcheng Zhan, Yanhua Chen, Hongmin Zhou, Ding Liu and 6 more

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Article in BMC cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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16 authors.

Shuai Liu *Department of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Heng Cao *Department of Urology, The Third the People's Hospital of Bengbu, Bengbu Medical College, Bengbu, China.
Bowen Chen *Department of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Lei JiangDepartment of Ophthalmology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.
Yimingniyizi NueraihemaitiDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Yuchen GaoDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Xiangcheng ZhanDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Yanhua ChenDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Hongmin ZhouDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Ding LiuDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Hao ChenDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Xiao XuDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Tiancheng XieDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China.
Wangli MeiDepartment of Urology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China. 1132792558@qq.com.
Yunze DongDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China. dongyz0277@qq.com.
Yunfei XuDepartment of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, China. xuyunfeish@163.com.

Funding

the National Natural Science Foundation of China 82101671
6 · The paper itself

Abstract

backgroundDysregulation of tRNA-derived small RNAs (tsRNAs) in various cancers has been indicated to play vital roles in tumorigenesis, but few reported in clear cell renal cell carcinoma (ccRCC). Here, we determined to elucidate the role of tsRNAs in ccRCC and their potential as new tumor biomarkers.

methodsWe obtained the tsRNA expression spectrum of ccRCC by a small RNA microarray sequence. Eight dysregulated tsRNAs were selected and validated by reverse transcription-quantitative real-time PCR (RT-qPCR). We identified these tsRNAs' potential target genes. The biological functions of tsRNAs were identified by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) assay.

resultsThe microarray sequence result shown statistically significantly 433 up-regulated tsRNAs and 798 down-regulated tsRNAs in ccRCC. Then, eight tsRNAs were validated by RT-qPCR and the target genes of the validated tsRNAs were predicted by TargetScan and miRanda databases. GO annotation and KEGG pathway enrichment analyses shown that potential biological functions and signaling pathways of the predicted target genes of tsRNAs in ccRCC. The results of external databases validation suggests that tRF5-23-ValAAC-2 may be a key biomarker for ccRCC development. The functional assays revealed that tRF5-23-ValAAC-2 hinders the proliferation and migration while promotes apoptosis of ccRCC.

conclusionsIn this study, we identified the tsRNA spectrum in ccRCC tissues and found that dysregulated tsRNAs may be novel biomarkers and possible therapeutic targets for ccRCC.

Indexed as

Biomarkers, TumorCarcinoma, Renal CellGene Expression Regulation, NeoplasticKidney NeoplasmsRNA, Small UntranslatedRNA, TransferCell Line, TumorCell MovementCell ProliferationGene Expression ProfilingHumansBiomarkers, TumorRNA, Small UntranslatedRNA, TransferBiomarkersClear cell renal cell carcinomaTsRNAs

Identifiers

PMID41029503
PMCPMC12482652

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.