Evidence map›Paper›PMID 41029365›Full record

ArticleBMC biology2025

Modification of adipose mass by targeting distal enhancers of Ptrf.

Yu Zhang, Keren Long, Songling Zhang, Biwei Zhang, Xiaokai Li, Xuemin Li, Zhining Zhong, Ziqi Li, Qiuyue Chen, Penghao Li and 11 more

Abstract read
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In one paragraph

Article in BMC biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Yu Zhang *State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Keren Long *State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China. keren.long@sicau.edu.cn.
Songling Zhang *State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Biwei ZhangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Xiaokai LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Xuemin LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Zhining ZhongState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Ziqi LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Qiuyue ChenState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Penghao LiJinxin Research Institute for Reproductive Medicine and Genetics, Chengdu, 610066, Sichuan, China.
Li ChenChongqing Academy of Animal Sciences, Chongqing, 402460, China.
Jinwei ZhangChongqing Academy of Animal Sciences, Chongqing, 402460, China.
Jianlin YuanState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Anan JiangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Lu LuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Haohuan LiCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Long JinState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Liangpeng GeChongqing Academy of Animal Sciences, Chongqing, 402460, China.
Peng ShangAnimal Science College, Tibet Agriculture and Animal Husbandry University, Linzhi, 860000, China.
Xuewei LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China. xuewei.li@sicau.edu.cn.
Mingzhou LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China. mingzhou.li@sicau.edu.cn.

Funding

the National Key R & D Program of China 2020YFA0509500the National Key R & D Program of China 2021YFA0805903the National Key R & D Program of China 2021YFD1300800the National Natural Science Foundation of China 32072687the National Natural Science Foundation of China 32102512the National Natural Science Foundation of China 32225046the National Natural Science Foundation of China 32341050the National Natural Science Foundation of China 32372846the Sichuan Science and Technology Program 2021YFYZ0009the Sichuan Science and Technology Program 2021ZDZX0008
6 · The paper itself

Abstract

backgroundAdipose tissue plays a central role in systemically metabolic regulation. Polymerase I and transcript release factor (PTRF) is responsible for caveolae structure formation and plays a crucial role in lipid metabolism.

resultsWe investigated the possibility of modifying adipose mass by targeting the enhancers of Ptrf. Single-nucleus transcriptomic analysis revealed that the reduction in fat mass caused by Ptrf knockout is associated with impaired adipogenesis and differentiation, adipocyte metabolism, nutrient transportation, and altered regulatory network. We demonstrated a closely positive association between Ptrf transcription and adipose mass in mice. Then, circular chromosome conformation capture sequencing and ChIP-seq data of mature adipocytes identified six candidate enhancers (E1-E6) of Ptrf, and the dual-luciferase reporting system revealed the transcriptional activity of three active candidate enhancers (E1, E3, E5) in Human embryonic kidney 293 (HEK293) and mouse embryo fibroblast (3T3-L1) cells. During the adipogenic differentiation of 3T3-L1, the epigenetic perturbation of these enhancers with the dCas9-KRAB system demonstrated their important roles during adipogenesis, with inhibited Ptrf expression and decreased lipid droplets and triglyceride contents. Injecting lentivirus carrying the dCas9-KRAB system into the inguinal fat of mice downregulated Ptrf expression and decreased body weight, body adipose percentage, and adipocyte diameter in vivo. Additionally, we observed the close binding of Pparg and Cebpa to Ptrf-E1.

conclusionsTaken together, these findings identify the mechanism by which Ptrf deletion leads to reduced fat mass in mice, and indicate that adipose mass may be reduced by targeting cis-regulatory elements of key adipogenesis-related genes.

Indexed as

AdipogenesisAdipose TissueEnhancer Elements, GeneticMembrane Proteins3T3-L1 CellsAdipocytesAnimalsHEK293 CellsHumansMaleMiceMice, Inbred C57BLMembrane Proteins4C-seqChromatin interactionEnhancerPtrfSnRNA-seq

Identifiers

PMID41029365
PMCPMC12482881

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.