Evidence map›Paper›PMID 41028746›Full record

ArticleNPJ science of food2025

Tracing the botanical origins of UK heather honey by relative quantification of plant DNA.

Sophie Dodd, Zoltan Kevei, Zahra Karimi, Jane Jennifer Suresh Kumar, Anastasios Koidis, Maria Anastasiadi

Abstract read
In one paragraph

Article in NPJ science of food, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sophie DoddCentre for Soil, Agrifood and Biosciences, Faculty of Engineering and Applied Sciences, Cranfield University, Cranfield, UK.
Zoltan KeveiCentre for Soil, Agrifood and Biosciences, Faculty of Engineering and Applied Sciences, Cranfield University, Cranfield, UK.
Zahra KarimiCentre for Soil, Agrifood and Biosciences, Faculty of Engineering and Applied Sciences, Cranfield University, Cranfield, UK.
Jane Jennifer Suresh KumarCentre for Soil, Agrifood and Biosciences, Faculty of Engineering and Applied Sciences, Cranfield University, Cranfield, UK.
Anastasios KoidisInstitute for Global Food Security, School of Biological Sciences, Queen's University of Belfast, Belfast, UK.
Maria AnastasiadiCentre for Soil, Agrifood and Biosciences, Faculty of Engineering and Applied Sciences, Cranfield University, Cranfield, UK. m.anastasiadi@cranfield.ac.uk.

Funding

UKRI BBRSC FoodBioSystems DTP BB/T008776/1
6 · The paper itself

Abstract

Heather honey is an important honey type produced in the UK, valued for its unique flavour, thixotropic texture and health-promoting properties. Botanical authentication can be challenging due to the natural variability in honey composition and typical pollen analysis relies heavily on expert knowledge. As an alternative, real-time PCR (qPCR) can be a rapid and robust method to identify floral species in honey. In this work, species-specific markers for Calluna vulgaris and Erica cinerea were developed and used to quantify 266 honey samples relative to the plant trnL P6 loop. The method classed 96% of 234 heather honeys as containing > 3% heather DNA, with 68% classified as dominant (> 45%) for ling heather origin. Moreover, high specificity was achieved with negligible amplification in the 32 non-heather honeys. Our qPCR method offered comparable results to melissopalynology, DNA metabarcoding, and digital PCR, showing potential as an alternative and accessible method for botanical authentication of heather honeys.

Identifiers

PMID41028746
PMCPMC12485031

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.