Evidence map›Paper›PMID 41028706›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Luciferase Complementation Imaging Assay (LCI) to Reveal the Interaction Between SPL7 and RAN1 Genes During Ethylene Signaling.

Yanzhi Yang, Lei Li

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Yanzhi YangPeking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Shandong, China. daphneyang@pku.edu.cn.
Lei LiPeking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Shandong, China. lei.li@pku-iaas.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The gaseous hormone ethylene regulates multiple aspects of plant growth and development, as well as responses to biotic and abiotic factors. The copper transporter RAN1 mediates the delivery of copper ions to ETR1 and is crucial for proper ethylene receptor function and thus proper ethylene signaling. Moreover, the central regulator of copper homeostasis, SPL7 interacts with RAN1 at the endoplasmic reticulum to modulate ethylene signaling. Here, we present a detailed protocol of luciferase complementation imaging (LCI), a bioluminescence technique, to examine the interaction between membrane-bound proteins, such as SPL7 and RAN1, in ethylene signaling.

Indexed as

ArabidopsisArabidopsis ProteinsEthylenesLuciferasesLuminescent MeasurementsSignal TransductionGene Expression Regulation, PlantArabidopsis ProteinsethyleneEthylenesLuciferasesAgrobacterium tumefaciensEthyleneInteractionLuciferase complementation imagingNicotiana benthamiana

Identifiers

PMID41028706

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.