Evidence map›Paper›PMID 41028704›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

GCN2 and EIN2 Pathway Activation Assays in Arabidopsis Under Hypoxia.

Hsing-Yi Cho, Mei-Yi Chou, Ming-Che Shih

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hsing-Yi ChoDepartment of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, USA. hycho@molbio.mgh.harvard.edu.
Mei-Yi ChouAgricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
Ming-Che ShihAgricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

General translational repression is a crucial process that reduces energy consumption under hypoxia. Ethylene can stimulate both GCN2 and EIN2 pathways to counteract this repression and facilitate adaptation to hypoxia upon submergence. Here, we present three rapid assays to investigate the activation of GCN2 and EIN2 pathways in translation under submergence in Arabidopsis, including the survival test, eIF2α phosphorylation detection, and newly synthesized protein quantification. These methodologies will contribute to understanding ethylene signaling and plant adaptation to hypoxia stress.

Indexed as

ArabidopsisArabidopsis ProteinsProtein Serine-Threonine KinasesSignal TransductionEthylenesEukaryotic Initiation Factor-2Gene Expression Regulation, PlantHypoxiaPhosphorylationProtein BiosynthesisProtein KinasesReceptors, Cell SurfaceArabidopsis ProteinsEIN2 protein, ArabidopsisethyleneEthylenesEukaryotic Initiation Factor-2GCN2 protein, ArabidopsisProtein KinasesProtein Serine-Threonine KinasesReceptors, Cell SurfaceEIN2EthyleneGCN2HypoxiaSubmergenceTranslation

Identifiers

PMID41028704

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.