Isolation and Characterization of Proximal Promoters Regulating Ethylene-Induced Ripening in Banana.

Devesh Shukla, Ravi Kesari, Prabodh Kumar Trivedi, Pravendra Nath

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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4 authors.

Devesh ShuklaTissue Culture and Transformation Facility, Plant Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.

Ravi KesariDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bihar, India.

Prabodh Kumar TrivediTissue Culture and Transformation Facility, Plant Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India. prabodht.cimap@csir.res.in.

Pravendra NathCSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fruit ripening is a key developmental phase with biochemical and physiological changes. In climacteric fruits (ethylene responsive), such as bananas, ethylene predominantly regulates these changes. This study presents a protocol to isolate and functionally analyse proximal promoters of banana ripening genes. Using genome walking, we identify promoter fragments of key ripening genes. We then characterize their ethylene responsiveness. Promoter-GUS fusion constructs are prepared for functional testing. We deliver these constructs transiently into banana fruit tissues. Promoter activity is quantified by histochemical (color-based) and fluorometric (fluorescence-based) β-glucuronidase (GUS) assays. Findings clarify the regulation of ripening-associated pathways. Therefore, they suggest genetic engineering options to extend shelf life and improve banana nutritional quality. This protocol provides a practical guide for researchers to study and manipulate the molecular mechanisms underlying fruit ripening.

Indexed as

EthylenesFruitMusaPromoter Regions, GeneticGene Expression Regulation, PlantPlants, Genetically ModifiedethyleneEthylenesBananaEthyleneFruit ripeningGenetic engineeringGenome walkingPromoter isolationTransient expression

Identifiers

PMID41028701

DOI10.1007/BF02712670

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.