ArticleMethods in molecular biology (Clifton, N.J.)2025
Estimation of 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Deaminase Enzyme in Bacteria.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Ethylene at a low concentration is essential for the germination of seeds and the development of seedlings in the plant species. However, the same ethylene at a higher concentration inhibits the elongation of roots, seed germination, and seedling development. In plants, 1-aminocyclopropane-1-carboxylic acid (ACC) serves as the direct precursor for the production of ethylene. The enzyme ACC deaminase produced by certain bacteria plays a pivotal role in reducing ethylene levels in plants by breaking down ACC into α-ketobutyrate and ammonia, thus positively influencing various physiological processes. Bacteria can utilize these compounds for their own growth and development. This chapter provides a comprehensive overview of quantitative as well as qualitative methods including colorimetric assay, solid plate, and PCR-based assays for readily screening unknown ACC deaminase-producing bacterial strains.
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