Methods to Isolate Enteric Glia from hPSC-Derived Enteric Ganglioids.

Evgeniia Pimenova, Faranak Fattahi, Homa Majd

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Evgeniia PimenovaDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.

Faranak FattahiDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. faranak.fattahi@ucsf.edu.

Homa MajdDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. homa.majd@ucsf.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enteric glia play pivotal roles in maintaining the enteric nervous system (ENS) homeostasis, supporting neuronal functions, and initiating and developing enteric neuropathies. However, understanding their diversity and functional roles has been formidably challenging due to difficulties in isolating these cells from human tissue. Here, we describe a detailed method for generating enteric glia from human pluripotent stem cells (hPSCs). This approach involves differentiating hPSCs into enteric glial cells through a vagal and enteric neural crest intermediates, replicating in vivo developmental processes. The hPSC-derived enteric glia express canonical markers (SOX10, GFAP, PLP1, S100B) and display the cellular diversity of primary human enteric glia, confirmed by single-nucleus RNA sequencing (snRNA-seq). These cultures are scalable, enabling high-throughput screening and molecular analysis, and they facilitate the study of glial interactions with other cell types, including immune cells, epithelial cells, and the gut microbiome. hPSC-derived enteric glia represent a significant advancement in enteric neurobiology, providing a versatile platform for disease modeling and therapeutic discovery, thereby improving our understanding of enteric glial cell functions in both health and disease.

Indexed as

Cell Culture TechniquesEnteric Nervous SystemNeurogliaPluripotent Stem CellsBiomarkersCell DifferentiationCells, CulturedHumansBiomarkersDGBIDrug screeningEnteric neuronsGliosisGutIn vitro differentiationPeripheral glia

Identifiers

PMID41028629

DOI10.1038/nature16951

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.