Evidence map›Paper›PMID 41028628›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Fluorescence-Activated Cell Sorting of Primary Enteric Glia.

Austin Irwin, Anthony Pellicano, Vladimir Grubišić

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Austin IrwinDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, Old Westbury, NY, USA.
Anthony PellicanoDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, Old Westbury, NY, USA.
Vladimir GrubišićDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, Old Westbury, NY, USA. Vladimir.Grubisic@nyit.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enteric glia are important regulators of the gastrointestinal homeostasis in health and disease. To better understand these processes, it is necessary to be able to isolate and study enteric glia. Here we provide a protocol that expands upon conventional methods of glial cell isolation with the addition of microfluidic cell sorting, exploiting the glia-specific expression of the ACSA2 surface protein. Isolated live cells can be used for a number of downstream processes, such as cell culturing and/or proteomic or genomic analysis. Thus, the detailed isolation methods used are potentially effective ways of isolating and enriching enteric glia.

Indexed as

Cell SeparationEnteric Nervous SystemFlow CytometryNeurogliaAnimalsCells, CulturedMiceACSA2Enteric glial cellsEnteric nervous system (ENS)Flow cytometryFluorescent activated cell sorting

Identifiers

PMID41028628

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.