Evidence map›Paper›PMID 41028625›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Differentiating Enteric Glial Cells Within Intestinal Organoids Derived from Human Pluripotent Stem Cells.

Simon Vales, Lola Bonneau, Maxime M Mahe

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Simon ValesUniversité de Nantes, Inserm, TENS, The Enteric Nervous System in Gut and Brain Diseases, IMAD, Nantes, France.
Lola BonneauCenter for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Maxime M MaheUniversité de Nantes, Inserm, TENS, The Enteric Nervous System in Gut and Brain Diseases, IMAD, Nantes, France. maxime.mahe@inserm.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human intestinal organoids (HIOs) derived from pluripotent stem cells are valuable tools for studying gastrointestinal development and function. Herein, we present a method for generating and co-culturing human intestinal organoids and enteric glial cells derived from human pluripotent stem cells. In addition, we describe how to collect and stain enteric glial cells within organoids embedded in Matrigel. The method outlines the precise steps required to successfully generate both human intestinal organoids and vagal neural crest cells, offering valuable insight into the study of human enteric glial cells.

Indexed as

Cell DifferentiationEnteric Nervous SystemIntestinesNeurogliaOrganoidsPluripotent Stem CellsCell Culture TechniquesCoculture TechniquesCollagenDrug CombinationsHumansLamininNeural CrestProteoglycansCollagenDrug CombinationsLamininmatrigelProteoglycansEnteric glial cellsHuman intestinal organoidsPluripotent stem cellsStepwise differentiationVagal neural crest cells

Identifiers

PMID41028625

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.