ArticleMethods in molecular biology (Clifton, N.J.)2026
Oligo-dT Anchored cDNA SCoT: An Innovative Approach for Deciphering Fusarium Wilt Resistance-Related Differential Gene Expression in Chickpea.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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7 authors.
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Abstract
The cDNA start codon targeted marker (cDNA-SCoT) methodology is a unique, simple, quick, and efficient method for investigating differential gene expression. The differentially expressed fragments (DEFs) in resistant and susceptible cultivars were identified using cDNA-SCoT profiling. Sanger sequencing and the nBLAST homology search program were used to characterize the functional properties of DEFs. As a result, this methodology is innovative and would be utilized in future studies to identify potential functional genes, which can then be used in marker creation, marker-assisted selection, and uncovering the regulatory role of the relevant genes.
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