Spot Inoculation of Begomovirus by Single Whitefly (Bemisia tabaci) Transmission in Mungbean Using Microcage.

Shikha Dixit, Pandian Rajendran, Revanasiddappa, Bhimeshwari Sahu, J Sridhar, Ashish Marathe, Palaiyur Nanjappan Sivalingam

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Shikha DixitICAR-National Institute of Biotic Stress Management, Raipur, Chhattisgarh, India.

Pandian RajendranICAR-National Institute of Biotic Stress Management, Raipur, Chhattisgarh, India.

RevanasiddappaICAR-National Institute of Biotic Stress Management, Raipur, Chhattisgarh, India.

Bhimeshwari SahuICAR-National Institute of Biotic Stress Management, Raipur, Chhattisgarh, India.

J SridharICAR-National Institute of Biotic Stress Management, Raipur, Chhattisgarh, India.

Ashish MaratheICAR-National Institute of Biotic Stress Management, Raipur, Chhattisgarh, India.

Palaiyur Nanjappan SivalingamICAR-National Institute of Biotic Stress Management, Raipur, Chhattisgarh, India.

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6 · The paper itself

Abstract

The Begomovirus genus is a prominent group of viruses that are transmitted by the whitefly vector, Bemisia tabaci, and have been known to cause significant yield reductions in several economically important crops worldwide. Among these viruses, the mungbean yellow mosaic India virus is particularly problematic, causing substantial yield losses in mungbean and proving difficult to control. Therefore, developing new techniques to study the vector-virus systems involving begomoviruses is a research priority for mungbean and other crops affected by these viruses. In this chapter, we present a technique suitable for enhancing the acquisition and inoculation of begomoviruses using a single whitefly. By confining the whitefly in an environment that closely mimics its natural habitat, we are able to increase the transmission efficiency of the virus. This technique can be easily and efficiently replicated in other host-begomovirus systems for various studies.

Indexed as

BegomovirusHemipteraInsect VectorsPlant DiseasesVignaAnimalsBegomovirusBemisia tabaciMicrocageMungbeanSpot inoculationVirus transmissionWhitefly

Identifiers

PMID41028575

DOI10.1016/j.jviromet.2007.08.004

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