ArticleMethods in molecular biology (Clifton, N.J.)2026
RNA Interference Strategy for the Development of Okra (Abelmoschus esculentus L. Moench) Lines Resistant to Yellow Vein Mosaic Virus.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is a promising molecular biology approach for combating viral diseases. In this study, we present an interference strategy targeting the viral βC1 gene to develop resistant okra lines against yellow vein mosaic virus (YVMV). The methodology involves several key steps, including total DNA isolation from infected plants, PCR amplification of the target gene, design of a hairpin RNAi construct harboring the βC1 gene of the β satellite of Begomovirus and utilizing the pRNAi-LIC (CD3-1285) vector. Successful transformation of the construct into the highly susceptible okra cv. Salkeerthi could be achieved using an in planta method. Additionally, the stem loop primers designed could amplify the target small interfering RNA (siRNA) through thermal cycling. Screening of transgenic lines is performed under containment conditions, involving the controlled release of infected vectors. This chapter provides detailed insights into the methodology and procedures for performing RNAi for the development of virus-resistant okra (Abelmoschus esculentus).
Indexed as
Identifiers
41028574What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.