Evidence map›Paper›PMID 41028493›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Concentration and Purification of Proteins Produced by Pichia pastoris by TFF with Ceramic Membranes.

Fuensanta Verdú-Navarro, Juan A Moreno-Cid, Ana G Del Hierro, Xavier Garcia-Ortega, Ruth Ordóñez

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Fuensanta Verdú-NavarroBioprocess R&D Pilot Plant, Bionet, Fuente Álamo, Spain.
Juan A Moreno-CidBioprocess R&D Pilot Plant, Bionet, Fuente Álamo, Spain.
Ana G Del HierroBioprocess R&D Pilot Plant, Bionet, Fuente Álamo, Spain.
Xavier Garcia-OrtegaDepartment of Chemical, Biological and Environmental Engineering, Engineering School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Ruth OrdóñezBioprocess R&D Pilot Plant, Bionet, Fuente Álamo, Spain. ruth.ordonez@bionet.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tangential flow filtration is a common strategy to concentrate proteins. In the case of proteins produced by Komagataella phaffii, filtration is commonly based on cassette membranes. However, there is an alternative that is more affordable, durable, and resistant: ceramic membranes. This chapter describes the methodology to concentrate and purify K. phaffii secreted recombinant proteins using ceramic membranes as an alternative to cassettes in a tangential flow filtration system.

Indexed as

CeramicsFiltrationMembranes, ArtificialRecombinant ProteinsSaccharomycetalesMembranes, ArtificialRecombinant ProteinsCeramic membraneConcentrationKomagataella phaffiiPichia pastorisProteinPurificationTangential flow filtrationUltrafiltration

Identifiers

PMID41028493

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.