Best Practices for Protein Expression in Pichia pastoris Combining Design of Experiments with an Automated Platform for Parallelized Cultivation in Small-Scale Stirred Tank Bioreactors.

Holger Morschett, Joan Cortada-Garcia, Hauke Holm, Núria Adelantado, Karlheinz Flicker

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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5 · Who and what money

Authors and funding

5 authors.

Holger MorschettLonza AG, Visp, Switzerland. holger.morschett@lonza.com.

Joan Cortada-GarciaLonza AG, Visp, Switzerland.

Hauke HolmLonza AG, Visp, Switzerland.

Núria AdelantadoLonza AG, Visp, Switzerland.

Karlheinz FlickerLonza AG, Visp, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter provides practical guidance for studying the expression of proteins in the yeast Komagataella phaffii by using a commercial automated platform for parallelized cultivation in single-use stirred tank bioreactors. In addition to discussing the advantages and disadvantages of such a platform regarding scale, automatability, and single-use vessels, the main focus is on the presentation of a flexibly adaptable, operator-centric workflow for the use of the cultivation system. In particular, it includes highlighting typical pitfalls and sources of error in handling and culminates in the presentation of best practices to support the proper execution of such experiments. To complement, a generic guide to its use for strain characterization and process development using statistical design of experiments is suggested based on the parallelization capabilities of the system.

Indexed as

Batch Cell Culture TechniquesBioreactorsRecombinant ProteinsSaccharomycetalesGuidelines as TopicRecombinant ProteinsAutomationDesign of experimentsFed-batchKomagataella phaffiiOptimizationParallelized cultivationPichia pastorisProcess developmentSingle-use

Identifiers

PMID41028485

DOI10.1021/acsomega.0c02292

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.