Evidence map›Paper›PMID 41028481›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Adapting Komagataella phaffii Cultivation Media to REACH Regulations.

Julian Kopp, Alexander Pekarsky, Oliver Spadiut

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Julian KoppInstitute of Chemical, Environmental and Bioscience Engineering, Research Group Integrated Bioprocess Development, TU Wien, Vienna, Austria. julian.kopp@tuwien.ac.at.
Alexander PekarskyInstitute of Chemical, Environmental and Bioscience Engineering, Research Group Integrated Bioprocess Development, TU Wien, Vienna, Austria.
Oliver SpadiutInstitute of Chemical, Environmental and Bioscience Engineering, Research Group Integrated Bioprocess Development, TU Wien, Vienna, Austria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

REACH stands for "Registration, Evaluation, Authorization, and Restriction of Chemicals" and classifies certain substances as harmful to human health and the environment. Boric acid and cobalt dichloride, commonly used as boron (B) and cobalt (Co) sources in cultivation media for Komagataella phaffii, represent such harmful substances. However, B and Co support cellular growth, and yeast harbors a large number of co-containing enzymes. Even though cell physiology (up to 90 g/L) was not affected by the removal of boric acid and cobalt dichloride from the media, recombinant protein production varied when excluding these components from the cultivation media to comply with REACH regulations. Here, we describe how to analyze and potentially adapt a defined cultivation medium for K. phaffii bioreactor cultivations concerning current REACH regulations.

Indexed as

Culture MediaSaccharomycetalesBioreactorsBoric AcidsBoronCobaltRecombinant Proteinsboric acidBoric AcidsBoronCobaltCulture MediaRecombinant ProteinsboronBoron leakageCobaltFermentationGlass vesselKomagataella phaffiiPichia pastorisREACHStainless steel

Identifiers

PMID41028481

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.